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Determination of glycation biomarkers in human fingernails by isotope-dilution liquid chromatography tandem mass spectrometry (LC-MS/MS)

dc.rights.licenseopenen_US
dc.contributor.authorTESSIER, Frederic J
dc.contributor.authorORTIZ, Sarahi Jaramillo
dc.contributor.authorNGUYEN, Dinh Hieu
dc.contributor.authorMOHAMMEDI, Kamel
hal.structure.identifierBordeaux population health [BPH]
dc.contributor.authorDELCOURT, Cecile
hal.structure.identifierBordeaux population health [BPH]
dc.contributor.authorHELMER, Catherine
hal.structure.identifierBordeaux population health [BPH]
dc.contributor.authorGOFF, Melanie Le
dc.contributor.authorBOULANGER, Eric
hal.structure.identifierBordeaux population health [BPH]
dc.contributor.authorRIGALLEAU, Vincent
IDREF: 069788146
dc.contributor.authorHOWSAM, Michael
dc.date.accessioned2024-12-18T15:04:20Z
dc.date.available2024-12-18T15:04:20Z
dc.date.issued2025-01-30
dc.identifier.issn1873-3492en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/203988
dc.description.abstractEnGlycation is a non-enzymatic, post-translational modification of proteins which is elevated in several pathologies, notably diabetes. An early-stage glycation product, glycated hemoglobin (HbA1c), is used in the clinical management of diabetes, and advanced glycation end-products (AGEs) are implicated in the etiology of diabetic complications. Fingernail clippings contain a time-integrated repository of several metabolic processes during the preceding 3-5 months, are easily sampled, and various elements and molecules have been shown to remain stable within them for long periods without refrigeration. Building upon a few underexploited studies, we investigated fingernails as a non-invasive matrix to assess glycation using liquid chromatography-mass spectrometry to quantify ungual biomarkers of early- and advanced glycation (respectively furosine, as a fructose-lysine derivative, and two AGEs (N(ε)-carboxymethyllysine (CML) and N(ε)-carboxyethyllysine (CEL)). The method was appropriately validated and provided accurate and precise measurements of two amino acids and the glycation biomarkers. Sample storage at ± 25 °C for 12 months had no effect upon these analytes, and the method was applied to fingernails from 87 people with diabetes. There was a moderate, linear correlation between ungual furosine concentrations and HbA1c at the time of nail sampling (r(s) = 0.339, p = 0.0011). Among subjects for whom previous measurements were available, there was no correlation between ungual glycation and HbA1c measured > 3 months before nail sampling, indicating that ungual furosine reflects early-stage glycation over a similar period to HbA1c. This study provides further evidence, using modern analytical techniques, that fingernails offer the possibility to quantitatively and non-invasively assess glycation.
dc.language.isoENen_US
dc.subject.enBiomarkers
dc.subject.enDiabetes
dc.subject.enFingernails
dc.subject.enGlycation
dc.subject.enHbA1c
dc.subject.enMass spectrometry
dc.title.enDetermination of glycation biomarkers in human fingernails by isotope-dilution liquid chromatography tandem mass spectrometry (LC-MS/MS)
dc.title.alternativeClin Chim Actaen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.cca.2024.120036en_US
dc.subject.halSciences du Vivant [q-bio]/Santé publique et épidémiologieen_US
dc.identifier.pubmed39551231en_US
bordeaux.journalClinica Chimica Actaen_US
bordeaux.page120036en_US
bordeaux.volume566en_US
bordeaux.hal.laboratoriesBordeaux Population Health Research Center (BPH) - UMR 1219en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.teamLEHA_BPHen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-04846367
hal.version1
hal.date.transferred2024-12-18T15:04:22Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Clinica%20Chimica%20Acta&rft.date=2025-01-30&rft.volume=566&rft.spage=120036&rft.epage=120036&rft.eissn=1873-3492&rft.issn=1873-3492&rft.au=TESSIER,%20Frederic%20J&ORTIZ,%20Sarahi%20Jaramillo&NGUYEN,%20Dinh%20Hieu&MOHAMMEDI,%20Kamel&DELCOURT,%20Cecile&rft.genre=article


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