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The Actin Depolymerizing Factor StADF2 Alters StREM1.3 Plasma Membrane Nanodomains to Inhibit the \emphPotato Virus X

hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorJOLIVET, Marie-Dominique
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
hal.structure.identifierEberhard Karls Universität Tübingen = University of Tübingen
dc.contributor.authorGOUGUET, Paul
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLEGRAND, Anthony
hal.structure.identifierEberhard Karls Universität Tübingen = University of Tübingen
dc.contributor.authorXHELILAJ, Kaltra
hal.structure.identifierEberhard Karls Universität Tübingen = University of Tübingen
dc.contributor.authorFAISS, Natalie
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorMASSONI-LAPORTE, Aurélie
hal.structure.identifierBoRdeaux Institute in onCology [Inserm U1312 - BRIC]
dc.contributor.authorROBBE, Terezinha
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorSAGOT, Isabelle
hal.structure.identifierInstitut des Sciences des Plantes de Paris-Saclay [IPS2 (UMR_9213 / UMR_1403)]
dc.contributor.authorBOUDSOCQ, Marie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGERMAN-RETANA, Sylvie
hal.structure.identifierEberhard Karls Universität Tübingen = University of Tübingen
hal.structure.identifierRuhr University Bochum = Ruhr-Universität Bochum [RUB]
dc.contributor.authorÜSTÜN, Suayib
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLOQUET, Antoine
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorHABENSTEIN, Birgit
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorGERMAIN, Véronique
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorMONGRAND, Sébastien
hal.structure.identifierUniversität Zürich [Zürich] = University of Zurich [UZH]
hal.structure.identifierZentrum für Molekularbiologie der Pflanzen [ZMBP]
dc.contributor.authorGRONNIER, Julien
dc.date.issued2023
dc.description.abstractEnABSTRACT The dynamic regulation of the plasma membrane (PM) organization at the nanoscale emerged as a key element shaping the outcome of host-microbe interactions. Protein organization into nanodomains (ND) is often assumed to be linked to the activation of cellular processes. In contrast, we have previously shown that the phosphorylation of the Solanum tuberosum REM1.3 (StREM1.3) N-terminal domain disperses its native ND organization and promotes its inhibitory effect on Potato Virus X (PVX) cell-to-cell movement. Here, we show that the phosphorylation of StREM1.3 modify the chemical environment of numerous residues in its intrinsically-disordered N-terminal domain. We leveraged exploratory screens to identify potential phosphorylation-dependent interactors of StREM1.3. Herewith, we uncovered uncharacterized regulators of PVX cell-to-cell movement, linking StREM1.3 to autophagy, water channels and the actin cytoskeleton. We show that the Solanum tuberosum actin depolymerizing factors 2 (StADF2) alters StREM1.3 NDs and limits PVX cell-to-cell movement in a REMORIN-dependent manner. Mutating a conserved single residue reported to affect ADFs affinity to actin inhibits StADF2 effect on StREM1.3 ND organization and PVX cell-to-cell movement. These observations provide functional links between the organization of plant PM and the actin cytoskeleton and suggests that the alteration of StREM1.3 ND organization promotes plant anti-viral responses. We envision that analogous PM re-organization applies for additional signaling pathways in plants and in other organisms.
dc.language.isoen
dc.title.enThe Actin Depolymerizing Factor StADF2 Alters StREM1.3 Plasma Membrane Nanodomains to Inhibit the \emphPotato Virus X
dc.typeDocument de travail - Pré-publication
dc.typePrepublication/Preprint
dc.identifier.doi10.1101/2023.01.25.525625
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire
hal.identifierhal-04833207
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04833207v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2023&rft.au=JOLIVET,%20Marie-Dominique&GOUGUET,%20Paul&LEGRAND,%20Anthony&XHELILAJ,%20Kaltra&FAISS,%20Natalie&rft.genre=preprint&unknown


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