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Multiple deletions of candidate effector genes lead to the breakdown of partial grapevine resistance to downy mildew

hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorPAINEAU, Manon
hal.structure.identifierDepartment of Viticulture and Enology [Davis]
dc.contributor.authorMINIO, Andrea
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorMESTRE, Pere
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorFABRE, Frédéric
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorMAZET, Isabelle
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorCOUTURE, Carole
hal.structure.identifierInstitut de Génétique, Environnement et Protection des Plantes [IGEPP]
dc.contributor.authorLEGEAI, Fabrice
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorDUMARTINET, Thomas
hal.structure.identifierDepartment of Viticulture and Enology [Davis]
dc.contributor.authorCANTU, Dario
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDELMOTTE, François
dc.date.accessioned2024-12-11T03:01:46Z
dc.date.available2024-12-11T03:01:46Z
dc.date.issued2024-06-21
dc.identifier.issn0028-646X
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/203822
dc.description.abstractEnGrapevine downy mildew, caused by the oomycete Plasmopara viticola (P. viticola, Berk. & M. A. Curtis; Berl. & De Toni), is a global threat to Eurasian wine grapes Vitis vinifera. Although resistant grapevine varieties are becoming more accessible, P. viticola populations are rapidly evolving to overcome these resistances. We aimed to uncover avirulence genes related to Rpv3.1-mediated grapevine resistance. We sequenced the genomes and characterized the development of 136 P. viticola strains on resistant and sensitive grapevine cultivars. A genome-wide association study was conducted to identify genomic variations associated with resistant-breaking phenotypes. We identified a genomic region associated with the breakdown of Rpv3.1 grapevine resistance (avrRpv3.1 locus). A diploid-aware reassembly of the P. viticola INRA-Pv221 genome revealed structural variations in this locus, including a 30 kbp deletion. Virulent P. viticola strains displayed multiple deletions on both haplotypes at the avrRpv3.1 locus. These deletions involve two paralog genes coding for proteins with 800-900 amino acids and signal peptides. These proteins exhibited a structure featuring LWY-fold structural modules, common among oomycete effectors. When transiently expressed, these proteins induced cell death in grapevines carrying Rpv3.1 resistance, confirming their avirulence nature. This discovery sheds light on the genetic mechanisms enabling P. viticola to adapt to grapevine resistance, laying a foundation for developing strategies to manage this destructive crop pathogen.
dc.language.isoen
dc.publisherWiley
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enavirulence
dc.subject.eneffector
dc.subject.enGWAS
dc.subject.enoomycete
dc.subject.enPlasmopara viticola (grapevine downy mildew)
dc.subject.enresistance breakdown
dc.subject.enstructural variant
dc.title.enMultiple deletions of candidate effector genes lead to the breakdown of partial grapevine resistance to downy mildew
dc.typeArticle de revue
dc.identifier.doi10.1111/nph.19861
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalNew Phytologist
bordeaux.page1490-1505
bordeaux.volume243
bordeaux.hal.laboratoriesBioGeCo (Biodiversité Gènes & Communautés) - UMR 1202*
bordeaux.issue4
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-04818749
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04818749v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=New%20Phytologist&rft.date=2024-06-21&rft.volume=243&rft.issue=4&rft.spage=1490-1505&rft.epage=1490-1505&rft.eissn=0028-646X&rft.issn=0028-646X&rft.au=PAINEAU,%20Manon&MINIO,%20Andrea&MESTRE,%20Pere&FABRE,%20Fr%C3%A9d%C3%A9ric&MAZET,%20Isabelle&rft.genre=article


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