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Grapevine cell response to carbon deficiency requires transcriptome and methylome reprogramming

hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorBERGER, Margot
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGARCIA, Virginie
hal.structure.identifierDémarche intégrée pour l'obtention d'aliments de qualité [UMR QualiSud]
hal.structure.identifierPlantes et systèmes de culture horticoles [PSH]
dc.contributor.authorLACRAMPE, Nathalie
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorRUBIO, Bernadette
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDECROS, Guillaume
hal.structure.identifierPlateforme Bordeaux Metabolome
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPÉTRIACQ, Pierre
hal.structure.identifierPlateforme Bordeaux Metabolome
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFLANDIN, Amélie
hal.structure.identifierPlateforme Bordeaux Metabolome
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCASSAN, Cédric
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorHILBERT‐MASSON, Ghislaine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorCOLOMBIE, Sophie
hal.structure.identifierSucres & Échanges Végétaux-Environnement [Équipe du laboratoire EBI Poitiers] [SEVE]
dc.contributor.authorATANASSOVA, Rossitza
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGALLUSCI, Philippe
dc.date.issued2025
dc.identifier.issn2052-7276
dc.description.abstractEnSugar limitation has dramatic consequences on plant cells, which include cell metabolism and transcriptional reprogramming, and the recycling of cellular components to maintain fundamental cell functions. There is however no description of the contribution of epigenetic regulations to the adaptation of plant cells to limited carbon availability. We investigated this question using non-photosynthetic grapevine cells (Vitis vinifera, cv Cabernet Sauvignon) cultured in vitro with contrasted glucose concentrations. Sugar depletion in the culture medium led to a rapid cell growth arrest and a major metabolic shift that include the depletion in soluble sugar and total amino acids and modulation of the cell redox status. Consistently, flux modeling showed a dramatic slowdown of many pathways required for biomass accumulation such as cell wall and protein synthesis. Sugar depletion also resulted in a major transcriptional reprogramming, characterized by the induction of genes involved in photosynthesis, and the repression of those related to sucrose mobilization or cell cycle control. Similarly, the epigenetic landscape was deeply modified. Glucose-depleted cells showed a higher global DNA methylation level than those grown with glucose. Changes in DNA methylation mainly occurred at transposable elements, and at genes including some of those differentially expressed, consistent with an important role for methylation to the adaptation of cells to limited sugar availability. In addition, genes encoding histone modifiers were differentially expressed suggesting that additional epigenetic mechanisms may be at work in plant cells under carbon shortage.
dc.language.isoen
dc.publisherNature Publishing Group
dc.title.enGrapevine cell response to carbon deficiency requires transcriptome and methylome reprogramming
dc.typeArticle de revue
dc.identifier.doi10.1093/hr/uhae277
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Botanique
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
bordeaux.journalHorticulture research
bordeaux.pageuhae277
bordeaux.volume12
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-04791091
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04791091v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Horticulture%20research&rft.date=2025&rft.volume=12&rft.issue=1&rft.spage=uhae277&rft.epage=uhae277&rft.eissn=2052-7276&rft.issn=2052-7276&rft.au=BERGER,%20Margot&GARCIA,%20Virginie&LACRAMPE,%20Nathalie&RUBIO,%20Bernadette&DECROS,%20Guillaume&rft.genre=article


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