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Specific nanoprobe design for MRI: Targeting laminin in the blood-brain barrier to follow alteration due to neuroinflammation

dc.rights.licenseopenen_US
dc.contributor.authorZAPATA-ACEVEDO, Juan
dc.contributor.authorLOSADA-BARRAGÁN, Mónica
dc.contributor.authorOSMA, Johann
dc.contributor.authorCRUZ, Juan
dc.contributor.authorREIBER, Andreas
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorPETRY, Klaus
dc.contributor.authorCAILLARD, Amael
dc.contributor.authorSAULDUBOIS, Audrey
dc.contributor.authorLLAMOSA PÉREZ, Daniel
dc.contributor.authorMORILLO ZÁRATE, Aníbal José
dc.contributor.authorMUÑOZ, Sonia Bermúdez
dc.contributor.authorDAZA MORENO, Agustín
dc.contributor.authorSILVA, Rafaela
dc.contributor.authorINFANTE-DUARTE, Carmen
dc.contributor.authorCHAMORRO-CORAL, William
dc.contributor.authorGONZÁLEZ-REYES, Rodrigo
dc.contributor.authorVARGAS-SÁNCHEZ, Karina
dc.date.accessioned2024-11-14T14:27:48Z
dc.date.available2024-11-14T14:27:48Z
dc.date.issued2024-04-11
dc.identifier.issn1932-6203en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/203283
dc.description.abstractEnChronic neuroinflammation is characterized by increased blood-brain barrier (BBB) permeability, leading to molecular changes in the central nervous system that can be explored with biomarkers of active neuroinflammatory processes. Magnetic resonance imaging (MRI) has contributed to detecting lesions and permeability of the BBB. Ultra-small superparamagnetic particles of iron oxide (USPIO) are used as contrast agents to improve MRI observations. Therefore, we validate the interaction of peptide-88 with laminin, vectorized on USPIO, to explore BBB molecular alterations occurring during neuroinflammation as a potential tool for use in MRI. The specific labeling of NPS-P88 was verified in endothelial cells (hCMEC/D3) and astrocytes (T98G) under inflammation induced by interleukin 1β (IL-1β) for 3 and 24 hours. IL-1β for 3 hours in hCMEC/D3 cells increased their co-localization with NPS-P88, compared with controls. At 24 hours, no significant differences were observed between groups. In T98G cells, NPS-P88 showed similar nonspecific labeling among treatments. These results indicate that NPS-P88 has a higher affinity towards brain endothelial cells than astrocytes under inflammation. This affinity decreases over time with reduced laminin expression. In vivo results suggest that following a 30-minute post-injection, there is an increased presence of NPS-P88 in the blood and brain, diminishing over time. Lastly, EAE animals displayed a significant accumulation of NPS-P88 in MRI, primarily in the cortex, attributed to inflammation and disruption of the BBB. Altogether, these results revealed NPS-P88 as a biomarker to evaluate changes in the BBB due to neuroinflammation by MRI in biological models targeting laminin.
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enSpecific nanoprobe design for MRI: Targeting laminin in the blood-brain barrier to follow alteration due to neuroinflammation
dc.typeArticle de revueen_US
dc.identifier.doi10.1371/journal.pone.0302031en_US
dc.subject.halSciences du Vivant [q-bio]en_US
bordeaux.journalPLoS ONEen_US
bordeaux.pagee0302031en_US
bordeaux.volume19en_US
bordeaux.hal.laboratoriesCentre de Résonance Magnétique des Systèmes Biologiques (CRMSB) - UMR 5536en_US
bordeaux.issue4en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-04766025
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS%20ONE&rft.date=2024-04-11&rft.volume=19&rft.issue=4&rft.spage=e0302031&rft.epage=e0302031&rft.eissn=1932-6203&rft.issn=1932-6203&rft.au=ZAPATA-ACEVEDO,%20Juan&LOSADA-BARRAG%C3%81N,%20M%C3%B3nica&OSMA,%20Johann&CRUZ,%20Juan&REIBER,%20Andreas&rft.genre=article


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