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A radical containing micellar probe for assessing esterase enzymatic activity with ultra-low field Overhauser-enhanced magnetic resonance imaging

dc.rights.licenseopenen_US
dc.contributor.authorELKHANOUFI, Sabrina
dc.contributor.authorRAKHSHAN, Sahar
dc.contributor.authorNESPECA, Martin
dc.contributor.authorALBERTI, Diego
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBOUDRIES, Dahmane
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorPOKONG-TOUYAM, Joyce
dc.contributor.authorSTEFANIA, Rachele
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorPARZY, Elodie
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorMASSOT, Philippe
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorMELLET, Philippe
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorFRANCONI, Jean-Michel
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorTHIAUDIERE, Eric
dc.contributor.authorGENINATTI CRICH, Simonetta
dc.date.accessioned2024-11-13T15:15:49Z
dc.date.available2024-11-13T15:15:49Z
dc.date.issued2024-10-30
dc.identifier.issn2050-750Xen_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/203251
dc.description.abstractEnThe ability to track altered enzyme activity using a non-invasive imaging protocol is crucial for the early diagnosis of many diseases but is often challenging. Herein, we show that Overhauser magnetic resonance imaging (OMRI) can be used to monitor enzymatic conversion at an ultra-low field (206 μT) using a highly sensitive "off/on" probe with a nitroxide stable radical containing ester, named T2C12-T80. This TEMPO derivative containing probe forms stable electron paramagnetic resonance (EPR) silent micelles in water that are hydrolysed by esterases, thus yielding narrow EPR signals whose intensities correlate directly with specific enzymatic activity. The responsiveness of the probe to tumours, facilitated by increased esterase activity, was initially determined by comparing EPR signals measured upon incubation with 3T3 (healthy fibroblasts used as control), HepG2 (human hepatoma) and Hs766T (human pancreatic cancer cells) cell lysates and then with Hs766T and 3T3 living cells. Next, Overhauser MR images were detected on a phantom containing the probe and the esterases to show that the approach is well suited for being translated to the in vivo detection at the earth's magnetic field. Regarding detection sensitivity, ultra-low field OMRI (ULF-OMRI) is beneficial over OMRI at higher fields (e.g. 0.2 T) since Overhauser enhancements are significantly higher and the technique is safe in terms of the specific absorption rate.
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enA radical containing micellar probe for assessing esterase enzymatic activity with ultra-low field Overhauser-enhanced magnetic resonance imaging
dc.title.alternativeJ Mater Chem Ben_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1039/d4tb00639aen_US
dc.subject.halSciences du Vivant [q-bio]en_US
dc.identifier.pubmed39331028en_US
bordeaux.journalJournal of materials chemistry‎ Ben_US
bordeaux.page10923-10933en_US
bordeaux.volume12en_US
bordeaux.hal.laboratoriesCentre de Résonance Magnétique des Systèmes Biologiques (CRMSB) - UMR 5536en_US
bordeaux.issue42en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-04765948
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20materials%20chemistry%E2%80%8E%20B&rft.date=2024-10-30&rft.volume=12&rft.issue=42&rft.spage=10923-10933&rft.epage=10923-10933&rft.eissn=2050-750X&rft.issn=2050-750X&rft.au=ELKHANOUFI,%20Sabrina&RAKHSHAN,%20Sahar&NESPECA,%20Martin&ALBERTI,%20Diego&BOUDRIES,%20Dahmane&rft.genre=article


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