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hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorJANAKIRAMAN, Vignesh Narasimhan
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorCABANNE, Charlotte
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorDIERYCK, Wilfrid
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorHOCQUELLET, Agnès
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorJOUCLA, Gilles
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorLE SENECHAL, Caroline
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCHAIGNEPAIN, Stéphane
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorCOSTAGLIOLI, Patricia
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorSANTARELLI, Xavier
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorGARBAY, Bertrand
IDREF: 033777551
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorNOUBHANI, Abdelmajid
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2015
dc.identifier.issn0168-1656
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20188
dc.description.abstractEnHepcidin was first identified as an antimicrobial peptide present in human serum and urine. It was later demonstrated that hepcidin is the long-sought hormone that regulates iron homeostasis in mammals. Recombinant human Hepcidin-25 (Hepc25) was expressed in Pichia pastoris using a modified version of the pPICZ alpha A vector. Hepc25 was then purified by a simple two-step chromatographic process to obtain 1.9 mg of soluble recombinant human Hepc25 per liter of culture at 96% purity. The sequence of Hepc25 and the presence of four disulfide bridges were confirmed by mass spectrometry analyses, and the recombinant Hepc25 exhibited antibacterial activity. This protocol of production and purification is the first step toward the production of human Hepc25 at a greater scale. (C) 2015 Elsevier B.V. All rights reserved.
dc.language.isoen
dc.publisherElsevier
dc.subject.enIMAC
dc.subject.enHepcidin
dc.subject.enP. pastoris
dc.subject.enRecombinant expression
dc.subject.enExpanded bed adsorption
dc.title.enProduction and purification of recombinant human hepcidin-25 with authentic N and C-termini
dc.typeArticle de revue
dc.identifier.doi10.1016/j.jbiotec.2014.12.025
dc.subject.halChimie/Polymères
bordeaux.journalJournal of Biotechnology
bordeaux.page89-92
bordeaux.volume195
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-01373236
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01373236v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Biotechnology&rft.date=2015&rft.volume=195&rft.spage=89-92&rft.epage=89-92&rft.eissn=0168-1656&rft.issn=0168-1656&rft.au=JANAKIRAMAN,%20Vignesh%20Narasimhan&CABANNE,%20Charlotte&DIERYCK,%20Wilfrid&HOCQUELLET,%20Agn%C3%A8s&JOUCLA,%20Gilles&rft.genre=article


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