HbIDI, SlIDI and EcIDI: A comparative study of isopentenyl diphosphate isomerase activity and structure
BALDERA-AGUAYO, Pedro A.
Columbia Univ, Dept Syst Biol
Columbia Univ, Integrated Program Cellular Mol & Biomed Studies
Univ Peruana Cayetano Heredia, Fac Ciencias & Filosofia
Columbia Univ, Dept Syst Biol
Columbia Univ, Integrated Program Cellular Mol & Biomed Studies
Univ Peruana Cayetano Heredia, Fac Ciencias & Filosofia
PERUCH, Frédéric
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 1 LCPO : Polymerization Catalyses & Engineering
< Réduire
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 1 LCPO : Polymerization Catalyses & Engineering
Langue
en
Article de revue
Ce document a été publié dans
Biochimie. 2016, vol. 127, p. 133-143
Elsevier
Résumé en anglais
In this study, we cloned, expressed and purified the isopentenyl diphosphate isomerases (IDIs) from two plants, Hevea brasiliensis and Solanum lycopersicum, and compared them to the already well characterized Escherichia ...Lire la suite >
In this study, we cloned, expressed and purified the isopentenyl diphosphate isomerases (IDIs) from two plants, Hevea brasiliensis and Solanum lycopersicum, and compared them to the already well characterized Escherichia coli IDI. Phylogenetic analysis showed high homology between the three enzymes. Their catalytic activity was investigated in vitro with recombinant purified enzymes and in vivo by complementation colorimetric tests. The three enzymes displayed consistent activities both in vitro and in vivo. In term of structure, studied by ATR-FTIR and molecular modeling, it is clear that both plant enzymes are more related to their human homologue than to E. coli IDI. But it is assumed that EcIDI represent the minimalistic part of the catalytic core, as both plant enzymes present a supplementary sequence forming an extra alpha-helice surrounding the catalytic site that could facilitate the biocatalysis. New potential biotechnological applications may be envisaged. (C) 2016 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.< Réduire
Mots clés en anglais
PURIFICATION
CRYSTALLOGRAPHY
ISOPENTENYLPYROPHOSPHATE
Solanum lycopersicum
Hevea brasiliensis
Enzyme structure-activity
CRYSTAL-STRUCTURE
Isoprenoid biosynthesis
Isopentenyl diphosphate isomerase
PYROPHOSPHATE ISOMERASE
CATALYTIC MECHANISM
ROLES
EARLY STEPS
ATR-FTIR
PLANT ISOPRENOID BIOSYNTHESIS
ESCHERICHIA-COLI
Origine
Importé de hal