Hepatitis B surface antigen reduction is associated with hepatitis B core-specific CD8+ T cell quality
| dc.rights.license | open | en_US |
| dc.contributor.author | TAKAHAMA, S. | |
| dc.contributor.author | YOSHIO, S. | |
| dc.contributor.author | MASUTA, Y. | |
| dc.contributor.author | MURAKAMI, H. | |
| dc.contributor.author | SAKAMORI, R. | |
| dc.contributor.author | KANEKO, S. | |
| dc.contributor.author | HONDA, T. | |
| dc.contributor.author | MURAKAWA, M. | |
| dc.contributor.author | SUGIYAMA, M. | |
| dc.contributor.author | KUROSAKI, M. | |
| dc.contributor.author | ASAHINA, Y. | |
| dc.contributor.author | TAKEHARA, T. | |
| hal.structure.identifier | Immunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept] | |
| dc.contributor.author | APPAY, Victor | |
| dc.contributor.author | KANTO, T. | |
| dc.contributor.author | YAMAMOTO, T. | |
| dc.date.accessioned | 2024-09-25T13:18:09Z | |
| dc.date.available | 2024-09-25T13:18:09Z | |
| dc.date.issued | 2023 | |
| dc.identifier.issn | 1664-3224 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/201808 | |
| dc.description.abstractEn | Despite treatment, hepatitis B surface antigen (HBsAg) persists in patients with chronic hepatitis B (CHB), suggesting the likely presence of the virus in the body. CD8+ T cell responses are essential for managing viral replication, but their effect on HBsAg levels remains unclear. We studied the traits of activated CD8+ T cells and HBV-specific CD8+ T cells in the blood of CHB patients undergoing nucleos(t)ide analog (NUC) therapy. For the transcriptome profiling of activated CD8+ T cells in peripheral blood mononuclear cells (PBMCs), CD69+ CD8+ T cells were sorted from six donors, and single-cell RNA sequencing (scRNA-seq) analysis was performed. To detect HBV-specific CD8+ T cells, we stimulated PBMCs from 26 donors with overlapping peptides covering the HBs, HBcore, and HBpol regions of genotype A/B/C viruses, cultured for 10 days, and analyzed via multicolor flow cytometry. scRNA-seq data revealed that CD8+ T cell clusters harboring the transcripts involved in the cytolytic functions were frequently observed in donors with high HBsAg levels. Polyfunctional analysis of HBV-specific CD8+ T cells utilized by IFN-γ/TNFα/CD107A/CD137 revealed that HBcore-specific cells exhibited greater polyfunctionality, suggesting that the quality of HBV-specific CD8+ T cells varies among antigens. Moreover, a subset of HBcore-specific CD8+ T cells with lower cytolytic potential was inversely correlated with HBsAg level. Our results revealed a stimulant-dependent qualitative difference in HBV-specific CD8+ T cells in patients with CHB undergoing NUC therapy. Hence, the induction of HBcore-specific CD8+ T cells with lower cytolytic potential could be a new target for reducing HBsAg levels. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | Chronic hepatitis B | |
| dc.subject.en | HBsAg | |
| dc.subject.en | HBV-specific CD8 + T cells | |
| dc.subject.en | scRNA-seq | |
| dc.subject.en | Cytotoxicity | |
| dc.title.en | Hepatitis B surface antigen reduction is associated with hepatitis B core-specific CD8+ T cell quality | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.3389/fimmu.2023.1257113 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Immunologie | en_US |
| dc.identifier.pubmed | 37920475 | en_US |
| bordeaux.journal | Frontiers in immunology | en_US |
| bordeaux.volume | 14 | en_US |
| bordeaux.hal.laboratories | ImmunoConcEpT - UMR 5164 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-04709326 | |
| hal.version | 1 | |
| hal.date.transferred | 2024-09-25T13:18:14Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| dc.rights.cc | CC BY | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20immunology&rft.date=2023&rft.volume=14&rft.eissn=1664-3224&rft.issn=1664-3224&rft.au=TAKAHAMA,%20S.&YOSHIO,%20S.&MASUTA,%20Y.&MURAKAMI,%20H.&SAKAMORI,%20R.&rft.genre=article |
