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Trojan Horse Strategy for Non-invasive Interference of Clock Gene in the Oyster Crassostrea gigas

dc.rights.licenseopenen_US
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorPAYTON, Laura
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorPERRIGAULT, Mickael
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOURDINEAUD, Jean-Paul
dc.contributor.authorMARCEL, Anjara
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorMASSABUAU, Jean-Charles
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorTRAN, Damien
dc.date.accessioned2024-09-23T12:14:32Z
dc.date.available2024-09-23T12:14:32Z
dc.date.issued2017-07-03
dc.identifier.issn1436-2228en_US
dc.identifier.urioai:crossref.org:10.1007/s10126-017-9761-9
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/201739
dc.description.abstractEnRNA interference is a powerful method to inhibit specific gene expression. Recently, silencing target genes by feeding has been successfully carried out in nematodes, insects, and small aquatic organisms. A non-invasive feeding-based RNA interference is reported here for the first time in a mollusk bivalve, the pacific oyster Crassostrea gigas. In this Trojan horse strategy, the unicellular alga Heterocapsa triquetra is the food supply used as a vector to feed oysters with Escherichia coli strain HT115 engineered to express the double-stranded RNA targeting gene. To test the efficacy of the method, the Clock gene, a central gene of the circadian clock, was targeted for knockout. Results demonstrated specific and systemic efficiency of the Trojan horse strategy in reducing Clock mRNA abundance. Consequences of Clock disruption were observed in Clock-related genes (Bmal, Tim1, Per, Cry1, Cry2, Rev.-erb, and Ror) and triploid oysters were more sensitive than diploid to the interference. This non-invasive approach shows an involvement of the circadian clock in oyster bioaccumulation of toxins produced by the harmful alga Alexandrium minutum.
dc.language.isoENen_US
dc.sourcecrossref
dc.subject.enBiological rhythms
dc.subject.enClock gene
dc.subject.enHarmful algae
dc.subject.enRNA interference
dc.subject.enCrassostrea gigas
dc.subject.enFeeding
dc.title.enTrojan Horse Strategy for Non-invasive Interference of Clock Gene in the Oyster Crassostrea gigas
dc.typeArticle de revueen_US
dc.identifier.doi10.1007/s10126-017-9761-9en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalMarine Biotechnologyen_US
bordeaux.page361-371en_US
bordeaux.volume19en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue4en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEAen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-04705943
hal.version1
hal.date.transferred2024-09-23T12:14:35Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Marine%20Biotechnology&rft.date=2017-07-03&rft.volume=19&rft.issue=4&rft.spage=361-371&rft.epage=361-371&rft.eissn=1436-2228&rft.issn=1436-2228&rft.au=PAYTON,%20Laura&PERRIGAULT,%20Mickael&BOURDINEAUD,%20Jean-Paul&MARCEL,%20Anjara&MASSABUAU,%20Jean-Charles&rft.genre=article


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