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CRISPR/Cas9 in Mycoplasma gallisepticum: intraspecific evolution of the specificity of a bacterial defense correlated with a host shift

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorIPOUTCHA, Thomas
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorTSARMPOPOULOS, Iason
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGOURGUES, Geraldine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorRIDEAU, Fabien
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBABY, Vincent
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorARFI, Yonathan
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBLANCHARD, Alain
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLARTIGUE, Carole
hal.structure.identifierLaboratoire Bordelais de Recherche en Informatique [LaBRI]
dc.contributor.authorTHEBAULT, Patricia
hal.structure.identifierCentre for Ecology and Conservation
dc.contributor.authorBONNEAUD, Camille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
dc.date.conference2021-11-01
dc.description.abstractEnCRISPR/Cas systems are adaptive defense mechanisms against invading nucleic acids that are widely found in prokaryotes. Mollicutes are currently the bacteria with the smallest genomes in which CRISPR/Cas systems have been reported. Indeed, complete or degraded systems were found in the genome of 21 out of 52 representative Mollicutes species, and most of them belong to the Type II-A while others to the Type II-C. In this study, we characterized a representative CRISPR/Cas9 system of mycoplasmas previously identified in the bird pathogen Mycoplasma gallisepticum (Mgal). In silico analysis provided evidence of CRISPR system evolution between the strain S6 and CA06 isolated in chicken and North American songbirds (house finch), respectively. In vitro cleavage assays were undertaken to determine the specificity of MgalCas9 from the two Mgal strains regarding the Protospacer Adjacent Motif (PAM), an element that is critical for the self/non-self recognition of the defense system. Cleavage of a plasmid library with a target and a degenerated 7 nt PAM sequence resulted in a 5-position PAM consensus of NNAAT and NNGAD for strains S6 and CA06, respectively. In vivo interference assays confirmed this difference of specificity using plasmid transformation experiments. Phylogenetic analyses using different strains of Mgal showed a correlation between the intraspecific evolution of MgalCas9 and the host shift that happened in 1995 when Mgal jumped from poultry to house finch. We are currently trying to understand more precisely this evolution of specificity and the potential role of this in the emergence of Mgal in a new host.
dc.language.isoen
dc.title.enCRISPR/Cas9 in Mycoplasma gallisepticum: intraspecific evolution of the specificity of a bacterial defense correlated with a host shift
dc.typeCommunication dans un congrès
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie
bordeaux.conference.title23th Congress of the International Organization for Mycoplasmology (IOM) (Virtual)
bordeaux.countryIL
bordeaux.conference.cityTel Aviv
bordeaux.peerReviewedoui
hal.identifierhal-04694125
hal.version1
hal.invitednon
hal.proceedingsnon
hal.conference.end2021-11-04
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04694125v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=IPOUTCHA,%20Thomas&TSARMPOPOULOS,%20Iason&GOURGUES,%20Geraldine&RIDEAU,%20Fabien&BABY,%20Vincent&rft.genre=unknown


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