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Synergistic approach of PCR-based fragment length analysis and amplicon deep sequencing reveals rich diversity of S-alleles in sweet cherries from the Caucasian region of origin

hal.structure.identifierJulius Kühn-Institut [JKI]
dc.contributor.authorSCHRÖPFER, Susan
hal.structure.identifierJulius Kühn-Institut [JKI]
dc.contributor.authorSCHUSTER, Mirko
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorQUERO-GARCIA, José
hal.structure.identifierAtlantic Green S.L
dc.contributor.authorLÓPEZ-ORTEGA, Gregorio
hal.structure.identifierJulius Kühn-Institut [JKI]
dc.contributor.authorFLACHOWSKY, Henryk
dc.date.accessioned2024-09-03T02:04:37Z
dc.date.available2024-09-03T02:04:37Z
dc.date.issued2024-04-05
dc.identifier.issn1664-462X
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/201384
dc.description.abstractEn<div>Introduction:<p>The self-incompatibility system in sweet cherry (Prunus avium L.) prevents fertilization with own or genetically related pollen, and is genetically determined by the multi-allelic S-locus. Therefore, determining S-alleles is crucial for plant breeding and fruit production, as it enables the selection of compatible combinations of S-genotypes for successful pollination.</p><p>Methods: In this study, S-alleles were identified in a total of 260 genotypes from the Caucasian region, the species' center of origin. S-allele genotyping was conducted using PCR fragment length analysis with the standard marker PaConsI-F/R2 and reference genotypes, complemented by sequence analysis through amplicon deep sequencing.</p><p>Results and discussion: The genotypes collected from Azerbaijan and Turkey exhibit a high allelic richness at the S-locus, particularly compared to modern sweet cherry cultivars worldwide. Nine previously undescribed S-alleles were identified and designated as S 45 , S 46 , S 47 , S 48 , S 49 , S 50 , S 51 , S 52 and S 53 . Given the expected high diversity for other traits, this plant material represents a valuable resource for further breeding research and introgression of new traits in future breeding programs. Furthermore, our results underscore that fragment length alone may not be sufficient for unambiguous assignment of S-alleles due to minimal length differences between different alleles. To address this issue, an Sallele reference ladder was developed using the rich diversity for precise</p></div>
dc.language.isoen
dc.publisherFrontiers
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enS-alleles
dc.subject.ensweet cherry
dc.subject.engenotyping
dc.subject.endiversity
dc.subject.enamplicon-deep sequencing
dc.subject.enfragment length analysis
dc.subject.enCaucasus
dc.subject.enS-allele reference collection
dc.title.enSynergistic approach of PCR-based fragment length analysis and amplicon deep sequencing reveals rich diversity of S-alleles in sweet cherries from the Caucasian region of origin
dc.typeArticle de revue
dc.identifier.doi10.3389/fpls.2024.1355977
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Amélioration des plantes
bordeaux.journalFrontiers in Plant Science
bordeaux.volume15
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-04684320
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04684320v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Frontiers%20in%20Plant%20Science&amp;rft.date=2024-04-05&amp;rft.volume=15&amp;rft.eissn=1664-462X&amp;rft.issn=1664-462X&amp;rft.au=SCHR%C3%96PFER,%20Susan&amp;SCHUSTER,%20Mirko&amp;QUERO-GARCIA,%20Jos%C3%A9&amp;L%C3%93PEZ-ORTEGA,%20Gregorio&amp;FLACHOWSKY,%20Henryk&amp;rft.genre=article


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