Afficher la notice abrégée

dc.rights.licenseopenen_US
dc.contributor.authorFADHLAOUI, Mariem
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorPIERRON, Fabien
hal.structure.identifierCentre Eau Terre Environnement [Québec] [INRS - ETE]
dc.contributor.authorCOUTURE, Patrice
dc.date.accessioned2024-09-02T16:11:44Z
dc.date.available2024-09-02T16:11:44Z
dc.date.issued2018-02
dc.identifier.issn0147-6513en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/201378
dc.description.abstractEnIn this study, we tested the hypothesis that metal exposure affected the normal thermal response of cell membrane FA composition and of elongase and desaturase gene transcription levels. To this end, muscle and brain membrane FA composition and FA desaturase (fads2, degs2 and scd2) and elongase (elovl2, elovl5 and elovl6) gene transcription levels were analyzed in fathead minnows (Pimephales promelas) acclimated for eight weeks to 15, 25 or 30 °C exposed or not to cadmium (Cd, 6 μg/l) or nickel (Ni, 450 6 μg/l). The response of membrane FA composition to temperature variations or metal exposure differed between muscle and brain. In muscle, an increase of temperature induced a decrease of polyunsaturated FA (PUFA) and an increase of saturated FA (SFA) in agreement with the current paradigm. Although a similar response was observed in brain between 15 and 25 °C, at 30 °C, brain membrane unsaturation was higher than predicted. In both tissues, metal exposure affected the normal thermal response of membrane FA composition. The transcription of desaturases and elongases was higher in the brain and varied with acclimation temperature and metal exposure but these variations did not generally reflect changes in membrane FA composition. The mismatch between gene transcription and membrane composition highlights that several levels of control other than gene transcription are involved in adjusting membrane FA composition, including post-transcriptional regulation of elongases and desaturases and de novo phospholipid biosynthesis. Our study also reveals that metal exposure affects the mechanisms involved in adjusting cell membrane FA composition in ectotherms.
dc.language.isoENen_US
dc.subject.enCell membranes
dc.subject.enTemperature
dc.subject.enMetals
dc.subject.enFatty acids
dc.subject.enDesaturases
dc.subject.enElongases
dc.title.enTemperature and metal exposure affect membrane fatty acid composition and transcription of desaturases and elongases in fathead minnow muscle and brain
dc.title.alternativeEcotoxicol Environ Safen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.ecoenv.2017.10.040en_US
dc.subject.halSciences du Vivant [q-bio]/Toxicologie/Ecotoxicologieen_US
dc.subject.halSciences de l'environnement/Milieux et Changements globauxen_US
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]en_US
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]en_US
bordeaux.journalEcotoxicology and Environmental Safetyen_US
bordeaux.page632-643en_US
bordeaux.volume148en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEAen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-02354058
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Ecotoxicology%20and%20Environmental%20Safety&rft.date=2018-02&rft.volume=148&rft.spage=632-643&rft.epage=632-643&rft.eissn=0147-6513&rft.issn=0147-6513&rft.au=FADHLAOUI,%20Mariem&PIERRON,%20Fabien&COUTURE,%20Patrice&rft.genre=article


Fichier(s) constituant ce document

Thumbnail

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée