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Impact of surface grafting density of PEG macromolecules on dually fluorescent silica nanoparticles used for the in vivo imaging of subcutaneous tumors

dc.rights.licenseopen
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorADUMEAU, Laurent
hal.structure.identifierImagerie moléculaire et thérapies innovantes en oncologie [IMOTION]
dc.contributor.authorGENEVOIS, Coralie
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorROUDIER, Lydia
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorSCHATZ, Christophe
hal.structure.identifierImagerie moléculaire et thérapies innovantes en oncologie [IMOTION]
dc.contributor.authorCOUILLAUD, Franck
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMORNET, Stéphane
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2017
dc.identifier.issn0304-4165
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20116
dc.description.abstractEnBackground: In the context of systematically administered nanomedicines, the physicochemistry of NP surfaces must be controlled as a prerequisite to improve blood circulation time, and passive and active targeting. In particular, there is a real need to develop NP stealth and labelling for both in vivo and microscopic fluorescence imaging in a mice model.Methods: We have synthesized NIR/red dually fluorescent silica nanoparticles of 19 nm covalently covered by a PEG layer of different grafting density in the brush conformational regime by using a reductive amination reaction. These particles were characterized by TEM, DRIFT, DLS, TGA, ζ potential measurements, UV-vis and fluorescence spectroscopy. Prostate tumors were generated in mice by subcutaneous injection of RM1-CMV-Fluc cells. Tumor growth was monitored by BLI after a D-luciferin injection. Four samples of PEGylated fluorescent NPs were individually intravenously injected into 6 mice (N = 6, total 24 mice). Nanoparticle distribution was investigated using in vivo fluorescence reflectance imaging (FRI) over 48 h and microscopy imaging was employed to localize the NPs within tumors in vitro.Results: Fluorescent NP accumulation, due to the enhanced permeability and retention (EPR) effect, increases gradually as a function of increased PEG surface grafting density with a huge difference observed for the highest density grafting. For the highest grafting density, a blood circulation time of up to 24 h was observed with a strong reduction in uptake by the liver. In vivo experimental results suggest that the biodistribution of NPs is very sensitive to slight variations in surface grafting density when the NPs present a high curvature radius.Conclusion: This study underlines the need to compensate a high curvature radius with a PEG-saturated NP surface to improve blood circulation and accumulation within tumors through the EPR effect. Dually fluorescent NPs PEGylated to saturation display physical properties useful for assessing the susceptibility of tumors to the EPR effect.
dc.description.sponsorshipTranslational Research and Advanced Imaging Laboratory - ANR-10-IDEX-03-02/10-LABX-0057
dc.language.isoen
dc.publisherElsevier
dc.subject.enbiodistribution
dc.subject.enin vivo
dc.subject.enEPR effect
dc.subject.enFluorescence imaging
dc.subject.enNanoparticle
dc.subject.enTumor
dc.subject.enPEGylation
dc.title.enImpact of surface grafting density of PEG macromolecules on dually fluorescent silica nanoparticles used for the in vivo imaging of subcutaneous tumors
dc.typeArticle de revue
dc.identifier.doi10.1016/j.bbagen.2017.01.036
dc.subject.halChimie/Matériaux
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale/Imagerie
bordeaux.journalBiochimica et Biophysica Acta (BBA) - General Subjects
bordeaux.page1587–1596
bordeaux.volume1861
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue6
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-01547431
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01547431v1
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