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HVA22a: a candidate protein involved in Potyvirus replication and movement

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorXUE, Mingshuo
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSOFER, Luc
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorVINCENT, Simon
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLION, Roxane
hal.structure.identifierUniversity of Saint Andrews
dc.contributor.authorTILSNER, Jens
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGERMAN-RETANA, Sylvie
dc.date.accessioned2024-08-02T02:03:56Z
dc.date.available2024-08-02T02:03:56Z
dc.date.conference2022-04-07
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/201146
dc.description.abstractEnThe potyvirus Turnip mosaic virus (TuMV) represents one rare example of phytoviruses that utilizes the plant endomembrane system to produce membranous vesicles mobile between cells through plasmodesmata (PD). The TuMV-6K2 protein induces rearrangements of the endoplasmic reticulum (ER) to generate those vesicles involved in viral replication and movement in the plant. In Arabidopsis thaliana, AtHVA22a (Hordeum vulgare abscisic acid responsive gene 22) belongs to a family of transmembrane proteins, homologous of interactors of reticulons (responsible for ER tubules constriction). Proteomics analysis showed that AtHVA22a is highly enriched in PD proteome. In our study, we showed that TuMV-6K2 interacts with AtHVA22a, by SuY2H in yeast and by BiFC in planta. The N-terminal half of the protein (including the transmembrane domains) of AtHVA22a is required for its interaction with 6K2. AtHVA22a is associated with cortical ER and partially to PD, and at the level of viral replication complex during TuMV infection, as well as the interaction between AtHAV22a and 6K2 observed in BiFC. Furthermore, overexpression of AtHVA22a increases TuMV propagation in Nicotiana benthamiana while TuMV propagation is slowed down in AtHVA22a-CRISPR/Cas9 mutants. Altogether, our results indicate that AtHVA22a plays an agonistic effect on TuMV propagation.
dc.description.sponsorshipFacteurs cellulaires recrutés par les potyvirus pour leur transport intercellulaire : de nouvelles sources de résistance des plantes? - ANR-16-CE20-0008
dc.language.isoen
dc.subject.enTuMV-6K2
dc.subject.enAtHVA22a
dc.subject.eninteraction
dc.subject.enpotyvirus movement
dc.title.enHVA22a: a candidate protein involved in Potyvirus replication and movement
dc.typeCommunication dans un congrès
dc.subject.halSciences du Vivant [q-bio]
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.conference.titleJournée de l'Ecole Doctorale des Sciences de la Vie et de la Santé de Bordeaux
bordeaux.countryFR
bordeaux.conference.cityBordeaux
bordeaux.peerReviewedoui
hal.identifierhal-04647984
hal.version1
hal.invitednon
hal.proceedingsnon
hal.conference.end2022-04-07
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04647984v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=XUE,%20Mingshuo&SOFER,%20Luc&VINCENT,%20Simon&LION,%20Roxane&TILSNER,%20Jens&rft.genre=unknown


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