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Improved CRISPR-base editor tools for genome edition in Mycoplasma bovis: Application to surface proteins

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorHOGAN, Patrick
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorRIDEAU, Fabien
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLARTIGUE, Carole
hal.structure.identifierGénomique, développement et pouvoir pathogène [GD2P]
dc.contributor.authorBLANCHARD, Alain
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
hal.structure.identifierGénomique, développement et pouvoir pathogène [GD2P]
dc.contributor.authorBEVEN, Laure
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorBARANOWSKI, Eric
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorARFI, Yonathan
dc.date.conference2024-07-07
dc.description.abstractEnAmong the minimal bacteria belonging to the genus Mycoplasma, several of them are recognized pathogens for a wide diversity of animals. Mycoplasma bovis is one of the most significant species infecting dairy and fattening cows, causing mastitis and pneumonia, respectively. Attempts to control the disease have led to mass culling and costs of hundreds of millions in treatments and compensation worldwide, emphasizing the need for improved vaccines. Within the RAMbo-V consortium (Rational Approach to a Mycoplasma bovis Vaccine), we believe that advances in genome engineering tools provide a unique opportunity to pave the way towards producing a vaccine strain of M. bovis expressing at its surface a specific set of conserved antigenic structures. Advances in CRISPR-based tools, such as SpyCas9 recognizing PAM variants and base editors, have allowed for an increase in potential uses. These technologies paired with an algorithm predicting the complete array of potential targets within a given CDS library of M. bovis has allowed for a more in-depth look at the possibilities of these technologies and the feasibility of gene knockouts. Here we report the use of multiple tools in M. bovis that led to the knockout of genes of interest such as the vsp (variable surface proteins) locus. VSPs are abundant and phase-variable components of the M. bovis membrane, which may participate in surface crowding and masking of stably expressed proteins, in addition to their putative biological functions. Targeting of the Xer1 tyrosine recombinase that permits spontaneous non-coordinate phase-variable expression results in the generation of phase-locked mutants. The development of genome engineering tools will permit targeting virulence factors and optimized antigenic presentation. Such tools will be useful to build a vaccine chassis that would allow presentation of selected epitopes at the cell surface, leading to an improved immune response.
dc.description.sponsorshipApproche rationnelle d'un vaccin Mycoplasma bovis - ANR-21-CE35-0008
dc.language.isoen
dc.title.enImproved CRISPR-base editor tools for genome edition in Mycoplasma bovis: Application to surface proteins
dc.typeCommunication dans un congrès
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie
bordeaux.conference.title25th Congres of the International Organization for Mycoplasmology
bordeaux.countryES
bordeaux.conference.cityLas Palmas De Gran Canaria
bordeaux.peerReviewedoui
hal.identifierhal-04654454
hal.version1
hal.invitednon
hal.proceedingsnon
hal.conference.end2024-07-11
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04654454v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=HOGAN,%20Patrick&RIDEAU,%20Fabien&LARTIGUE,%20Carole&BLANCHARD,%20Alain&SIRAND-PUGNET,%20Pascal&rft.genre=unknown


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