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Impact of house dust mite in vitiligo skin: environmental contribution to increased cutaneous immunity and melanocyte detachment

dc.rights.licenseopenen_US
dc.contributor.authorBZIOUECHE, H.
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorBONIFACE, Katia
IDREF: 10566913X
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorDRULLION, Claire
dc.contributor.authorMARCHETTI, S.
dc.contributor.authorCHIGNON-SICARD, B.
dc.contributor.authorSORMANI, L.
dc.contributor.authorROCCHI, S.
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorSENESCHAL, Julien
dc.contributor.authorPASSERON, T.
dc.contributor.authorTULIC, M.K.
dc.date.accessioned2024-07-05T08:17:53Z
dc.date.available2024-07-05T08:17:53Z
dc.date.issued2023-05
dc.identifier.issn0007-0963en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/200754
dc.description.abstractEnBackground: Vitiligo is an autoimmune skin disorder characterized by loss of melanocytes. Protease-mediated disruption of junctions between keratinocytes and/or keratinocyte intrinsic dysfunction may directly contribute to melanocyte loss. House dust mite (HDM), an environmental allergen with potent protease activity, contributes to respiratory and gut disease but also to atopic dermatitis and rosacea. Objectives: To verify if HDM can contribute to melanocyte detachment in vitiligo and if so, by which mechanism(s). Methods: Using primary human keratinocytes, human skin biopsies from healthy donors and patients with vitiligo, and 3D reconstructed human epidermis, we studied the effect of HDM on cutaneous immunity, tight and adherent junction expression and melanocyte detachment. Results: HDM increased keratinocyte production of vitiligo-associated cytokines and chemokines and increased expression of toll-like receptor (TLR)-4. This was associated with increased in situ matrix-metalloproteinase (MMP)-9 activity, reduced cutaneous expression of adherent protein E-cadherin, increased soluble E-cadherin in culture supernatant and significantly increased number of suprabasal melanocytes in the skin. This effect was dose-dependent and driven by cysteine protease Der p1 and MMP-9. Selective MMP-9 inhibitor, Ab142180, restored E-cadherin expression and inhibited HDM-induced melanocyte detachment. Keratinocytes from patients with vitiligo were more sensitive to HDM-induced changes than healthy keratinocytes. All results were confirmed in a 3D model of healthy skin and in human skin biopsies. Conclusions: Our results highlight that environmental mite may act as an external source of pathogen-associated molecular pattern molecules in vitiligo and topical MMP-9 inhibitors may be useful therapeutic targets. Whether HDM contributes to the onset of flares in vitiligo remains to be tested in carefully controlled trials.
dc.language.isoENen_US
dc.title.enImpact of house dust mite in vitiligo skin: environmental contribution to increased cutaneous immunity and melanocyte detachment
dc.typeArticle de revueen_US
dc.identifier.doi10.1093/bjd/ljad148en_US
dc.subject.halSciences du Vivant [q-bio]/Immunologieen_US
dc.identifier.pubmed37140010en_US
bordeaux.journalBritish Journal of Dermatologyen_US
bordeaux.page312-327en_US
bordeaux.volume189en_US
bordeaux.hal.laboratoriesImmunoConcEpT - UMR 5164en_US
bordeaux.issue3en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-04636326
hal.version1
hal.date.transferred2024-07-05T08:17:55Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
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