Afficher la notice abrégée

Asymmetric Hybrid Polymer-Lipid Giant Vesicles as Cell Membrane Mimics

dc.rights.licenseopen
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorPEYRET, Ariane
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorIBARBOURE, Emmanuel
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLE MEINS, Jean-Francois
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLECOMMANDOUX, Sebastien
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2018
dc.identifier.issn2198-3844
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20058
dc.description.abstractEnLipid membrane asymmetry plays an important role in cell function and activity, being for instance a relevant signal of its integrity. The development of artificial asymmetric membranes thus represents a key challenge. In this context, an emulsion-centrifugation method is developed to prepare giant vesicles with an asymmetric membrane composed of an inner monolayer of poly(butadiene)-b-poly(ethylene oxide) (PBut-b-PEO) and outer monolayer of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). The formation of a complete membrane asymmetry is demonstrated and its stability with time is followed by measuring lipid transverse diffusion. From fluorescence spectroscopy measurements, the lipid half-life is estimated to be 7.5 h. Using fluorescence recovery after photobleaching technique, the diffusion coefficient of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (DOPE-rhod, inserted into the POPC leaflet) is determined to be about D = 1.8 ± 0.50 μm2 s−1 at 25 °C and D = 2.3 ± 0.7 μm2 s−1 at 37 °C, between the characteristic values of pure POPC and pure polymer giant vesicles and in good agreement with the diffusion of lipids in a variety of biological membranes. These results demonstrate the ability to prepare a cell-like model system that displays an asymmetric membrane with transverse and translational diffusion properties similar to that of biological cells.
dc.description.sponsorshipDéveloppement de cellule synthétique comme micro-réacteur pour l'étude de l'activité enzymatique des NO-synthases et la compréhension de leur fonctionnement en conditions physiologiques - ANR-14-CE16-0015
dc.language.isoen
dc.publisherWiley Open Access
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enAsymmetric Hybrid Polymer-Lipid Giant Vesicles as Cell Membrane Mimics
dc.typeArticle de revue
dc.identifier.doi10.1002/advs.201700453
dc.subject.halPhysique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
dc.subject.halChimie/Polymères
dc.subject.halChimie/Matériaux
bordeaux.journalAdvanced Science
bordeaux.page1700453
bordeaux.volume5
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue1
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-01659989
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01659989v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Advanced%20Science&rft.date=2018&rft.volume=5&rft.issue=1&rft.spage=1700453&rft.epage=1700453&rft.eissn=2198-3844&rft.issn=2198-3844&rft.au=PEYRET,%20Ariane&IBARBOURE,%20Emmanuel&LE%20MEINS,%20Jean-Francois&LECOMMANDOUX,%20Sebastien&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée