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AtHVA22a , a plant‐specific homologue of Reep/ DP1 /Yop1 family proteins is involved in turnip mosaic virus propagation

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorXUE, Mingshuo
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSOFER, Luc
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIMON, Vincent
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARISSAL-ARVY, Nathalie
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorDIOP, Mamoudou
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLION, Roxane
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEUCHER, Guillaume
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBORDAT, Amandine
hal.structure.identifierUniversity of St Andrews [Scotland]
dc.contributor.authorTILSNER, Jens
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorGALLOIS, Jean‐luc
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGERMAN‐RETANA, Sylvie
dc.date.issued2024-05-20
dc.identifier.issn1464-6722
dc.description.abstractEnThe movement of potyviruses, the largest genus of single‐stranded, positive‐sense RNA viruses responsible for serious diseases in crops, is very complex. As potyviruses developed strategies to hijack the host secretory pathway and plasmodesmata (PD) for their transport, the goal of this study was to identify membrane and/or PD‐proteins that interact with the 6K2 protein, a potyviral protein involved in replication and cell‐to‐cell movement of turnip mosaic virus (TuMV). Using split‐ubiquitin membrane yeast two‐hybrid assays, we screened an Arabidopsis cDNA library for interactors of TuMV 6K2. We isolated AtHVA22a ( Hordeum vulgare abscisic acid responsive gene 22 ), which belongs to a multigenic family of transmembrane proteins, homologous to Receptor expression‐enhancing protein (Reep)/Deleted in polyposis (DP1)/Yop1 family proteins in animal and yeast. HVA22/DP1/Yop1 family genes are widely distributed in eukaryotes, but the role of HVA22 proteins in plants is still not well known, although proteomics analysis of PD fractions purified from Arabidopsis suspension cells showed that AtHVA22a is highly enriched in a PD proteome. We confirmed the interaction between TuMV 6K2 and AtHVA22a in yeast, as well as in planta by using bimolecular fluorescence complementation and showed that TuMV 6K2/AtHVA22a interaction occurs at the level of the viral replication compartment during TuMV infection. Finally, we showed that the propagation of TuMV is increased when AtHVA22a is overexpressed in planta but slowed down upon mutagenesis of AtHVA22a by CRISPR‐Cas9. Altogether, our results indicate that AtHVA22a plays an agonistic effect on TuMV propagation and that the C‐terminal tail of the protein is important in this process.
dc.description.sponsorshipFacteurs cellulaires recrutés par les potyvirus pour leur transport intercellulaire : de nouvelles sources de résistance des plantes?
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004
dc.language.isoen
dc.publisherWiley
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.subject.en6K2 vesicles
dc.subject.enAtHVA22a
dc.subject.enendoplasmic reticulum
dc.subject.enmovement
dc.subject.enplasmodesmata
dc.subject.enpotyvirus
dc.subject.enpropagation
dc.subject.enviral replication compartment
dc.subject.en6K2 vesicles AtHVA22a endoplasmic reticulum movement plasmodesmata potyvirus propagation viral replication compartment
dc.title.enAtHVA22a , a plant‐specific homologue of Reep/ DP1 /Yop1 family proteins is involved in turnip mosaic virus propagation
dc.typeArticle de revue
dc.identifier.doi10.1111/mpp.13466
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalMolecular Plant Pathology
bordeaux.pagee13466
bordeaux.volume25
bordeaux.peerReviewedoui
hal.identifierhal-04617334
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04617334v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20Plant%20Pathology&rft.date=2024-05-20&rft.volume=25&rft.spage=e13466&rft.epage=e13466&rft.eissn=1464-6722&rft.issn=1464-6722&rft.au=XUE,%20Mingshuo&SOFER,%20Luc&SIMON,%20Vincent&MARISSAL-ARVY,%20Nathalie&DIOP,%20Mamoudou&rft.genre=article


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