Gene expression patterns and related enzymatic activities of detoxification and oxidative stress systems in zebrafish larvae exposed to the 2,4-dichlorophenoxyacetic acid herbicide.
| dc.rights.license | open | en_US |
| dc.contributor.author | GAAIED, Sonia | |
| dc.contributor.author | OLIVEIRA, Miguel | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | LE BIHANIC, Florane | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | CACHOT, Jérôme
ORCID: 0000-0002-1492-9684 IDREF: 19667672X | |
| dc.contributor.author | BANNI, Mohamed | |
| dc.date.accessioned | 2024-06-06T07:21:21Z | |
| dc.date.available | 2024-06-06T07:21:21Z | |
| dc.date.issued | 2019-06-01 | |
| dc.identifier.issn | 1879-1298 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/200293 | |
| dc.description.abstractEn | The present study aims to assess the effects of 2,4-D herbicide on biotransformation and oxidative stress status of zebrafish larvae. Animals were exposed to a range of sublethal concentrations (0.02-0.8 mg/L) and biomarkers at transcriptomic level and biochemical level were assessed. Chemical analysis with showed that the bioaccumulation of 2,4-D in 96 hpf zebrafish larvae were increased in a concentration-dependent manner. This herbicide induced significant effects at both gene expression and enzymatic activities levels after at 96 hpf. Results of mRNA expression showed a differential transcription regulation with all target genes depending on the tested concentrations. The mRNA level of gsr and cyp1a were up regulated at the highest dose of herbicide (0.8 mg/L). The gene expression of gstp1 showed an up regulation at lower dose (0.02 mg/L) and a down regulation at the highest dose (0.8 mg/L) of 2,4-D. A significant induction of EROD activity and inhibition of GST activity were noted in groups exposed to 0.8 mg/L of 2,4-D. Considering the antioxidant defenses, the activity of CAT was increased in larvae exposed to 0.8 mg/L of herbicide and GPx activity was induced at lower doses of 2,4-D (0.02 and 0.051 mg/L). Moreover, peroxidative damage, assessed as MDA content, was markedly increased in larvae exposed to high 2,4-D concentration. Overall, the present study data indicate that bioaccumulation of 2,4-D in 96 hpf zebrafish larvae and alterations in detoxification and oxidative stress related parameters, likely associated with ROS production, which may endanger the embryo-larval stages development of fish. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | 2 | |
| dc.subject.en | 4-Dichlorophenoxyacetic Acid | |
| dc.subject.en | Animals | |
| dc.subject.en | Antioxidants | |
| dc.subject.en | Embryo | |
| dc.subject.en | Nonmammalian | |
| dc.subject.en | Herbicides | |
| dc.subject.en | Inactivation | |
| dc.subject.en | Metabolic | |
| dc.subject.en | Larva | |
| dc.subject.en | Oxidative Stress | |
| dc.subject.en | Transcriptome | |
| dc.subject.en | Water Pollutants | |
| dc.subject.en | Chemical | |
| dc.subject.en | Zebrafish | |
| dc.title.en | Gene expression patterns and related enzymatic activities of detoxification and oxidative stress systems in zebrafish larvae exposed to the 2,4-dichlorophenoxyacetic acid herbicide. | |
| dc.title.alternative | Chemosphere | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.chemosphere.2019.02.125 | en_US |
| dc.subject.hal | Sciences de l'environnement | en_US |
| dc.identifier.pubmed | 30825855 | en_US |
| bordeaux.journal | Chemosphere | en_US |
| bordeaux.page | 289-297 | en_US |
| bordeaux.volume | 224 | en_US |
| bordeaux.hal.laboratories | EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.team | EA | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04602919 | |
| hal.version | 1 | |
| hal.date.transferred | 2024-06-06T07:21:23Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Chemosphere&rft.date=2019-06-01&rft.volume=224&rft.spage=289-297&rft.epage=289-297&rft.eissn=1879-1298&rft.issn=1879-1298&rft.au=GAAIED,%20Sonia&OLIVEIRA,%20Miguel&LE%20BIHANIC,%20Florane&CACHOT,%20J%C3%A9r%C3%B4me&BANNI,%20Mohamed&rft.genre=article |
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