Afficher la notice abrégée

In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia

dc.rights.licenseopen
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorSANDRE, Olivier
hal.structure.identifierImagerie moléculaire et thérapies innovantes en oncologie [IMOTION]
dc.contributor.authorGENEVOIS, Coralie
hal.structure.identifierUniversidad del Pais Vasco / Euskal Herriko Unibertsitatea [Espagne] [UPV/EHU]
dc.contributor.authorGARAIO, Eneko
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorADUMEAU, Laurent
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMORNET, Stéphane
hal.structure.identifierImagerie moléculaire et thérapies innovantes en oncologie [IMOTION]
dc.contributor.authorCOUILLAUD, Franck
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2017
dc.identifier.issn2073-4425
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/19968
dc.description.abstractEnThe present work aims to demonstrate that colloidal dispersions of magnetic iron oxide nanoparticles stabilized with dextran macromolecules placed in an alternating magnetic field can not only produce heat, but also that these particles could be used in vivo for local and noninvasive deposition of a thermal dose sufficient to trigger thermo-induced gene expression. Iron oxide nanoparticles were first characterized in vitro on a bio-inspired setup, and then they were assayed in vivo using a transgenic mouse strain expressing the luciferase reporter gene under transcriptional control of a thermosensitive promoter. Iron oxide nanoparticles dispersions were applied topically on the mouse skin or injected subcutaneously with Matrigel™ to generate so-called pseudotumors. Temperature was monitored continuously with a feedback loop to control the power of the magnetic field generator and to avoid overheating. Thermo-induced luciferase expression was followed by bioluminescence imaging 6 h after heating. We showed that dextran-coated magnetic iron oxide nanoparticle dispersions were able to induce in vivo mild hyperthermia compatible with thermo-induced gene expression in surrounding tissues and without impairing cell viability. These data open new therapeutic perspectives for using mild magnetic hyperthermia as noninvasive modulation of tumor microenvironment by local thermo-induced gene expression or drug release.
dc.description.sponsorshipMagnéto-Chimiothérapie : Modélisation de la Délivrance Induite par Champ Magnétique Radiofréquence d'Anticancéreux par des Nano-Vésicules Polymères et Suivi par IRM d'un Modèle de Glioblastome - ANR-13-BS08-0017
dc.description.sponsorshipTranslational Research and Advanced Imaging Laboratory - ANR-10-IDEX-03-02/10-LABX-0057
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enmagnetic polymer-coated nanoparticles
dc.subject.enin vivo optical imaging
dc.subject.enheat shock protein promoter
dc.subject.engene therapies
dc.subject.enmagnetic hyperthermia
dc.title.enIn Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia
dc.typeArticle de revue
dc.identifier.doi10.3390/genes8020061
dc.subject.halChimie/Matériaux
dc.subject.halChimie/Polymères
dc.subject.halPhysique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale
dc.description.sponsorshipEuropeMultifunctional Nanoparticles for Magnetic Hyperthermia and Indirect Radiation Therapy
bordeaux.journalGenes
bordeaux.page61
bordeaux.volume8
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue2
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-01462355
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01462355v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Genes&rft.date=2017&rft.volume=8&rft.issue=2&rft.spage=61&rft.epage=61&rft.eissn=2073-4425&rft.issn=2073-4425&rft.au=SANDRE,%20Olivier&GENEVOIS,%20Coralie&GARAIO,%20Eneko&ADUMEAU,%20Laurent&MORNET,%20St%C3%A9phane&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée