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High-Content Screening of Plankton Alkaline Phosphatase Activity in Microfluidics
| dc.rights.license | open | en_US |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | GIRAULT, Mathias | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | BENEYTON, Thomas | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | PEKIN, Deniz | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | BUISSON, Lionel | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | BICHON, Sabrina | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | CHARBONNIER, Celine
IDREF: 176318933 | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | DEL AMO, Yolanda | |
| hal.structure.identifier | Centre de Recherche Paul Pascal [CRPP] | |
| dc.contributor.author | BARET, Jean-Christophe
IDREF: 19204690X | |
| dc.date.accessioned | 2024-04-26T11:04:02Z | |
| dc.date.available | 2024-04-26T11:04:02Z | |
| dc.date.issued | 2018 | |
| dc.identifier.issn | 0003-2700 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/199353 | |
| dc.description.abstractEn | One way for phytoplankton to survive orthophosphate depletion is to utilize dissolved organic phosphorus by expressing alkaline phosphatase. The actual methods to assay alkaline phosphate activity—either in bulk or as a presence/absence of enzyme activity—fail to provide information on individual living cells. In this context, we develop a new microfluidic method to compartmentalize cells in 0.5 nL water-in-oil droplets and measure alkaline phosphatase activity at the single-cell level. We use enzyme-labeled fluorescence (ELF), which is based on the hydrolysis of ELF-P substrate, to monitor in real time and at the single-cell level both qualitative and quantitative information on cell physiology (i.e., localization and number of active enzyme sites and alkaline phosphatase kinetics). We assay the alkaline phosphatase activity of Tetraselmis sp. as a function of the dissolved inorganic phosphorus concentration and show that the time scale of the kinetics spans 1 order of magnitude. The advantages of subnanoliter-scale compartmentalization in droplet-based microfluidics provide a precise characterization of a population with single-cell resolution. Our results highlight the key role of cell physiology to efficiently access dissolved organic phosphorus. | |
| dc.description.sponsorship | Initiative d'excellence de l'Université de Bordeaux - ANR-10-IDEX-0003 | en_US |
| dc.language.iso | EN | en_US |
| dc.subject.en | Microfluic | |
| dc.subject.en | Vesicles | |
| dc.subject.en | Droplet | |
| dc.title.en | High-Content Screening of Plankton Alkaline Phosphatase Activity in Microfluidics | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1021/acs.analchem.8b00234 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM] | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie | en_US |
| dc.subject.hal | Sciences de l'environnement/Ingénierie de l'environnement | en_US |
| bordeaux.journal | Analytical Chemistry | en_US |
| bordeaux.page | 4174 - 4181 | en_US |
| bordeaux.volume | 90 | en_US |
| bordeaux.hal.laboratories | EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805 | en_US |
| bordeaux.issue | 6 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.team | ECOBIOC | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-01757331 | |
| hal.version | 1 | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analytical%20Chemistry&rft.date=2018&rft.volume=90&rft.issue=6&rft.spage=4174%20-%204181&rft.epage=4174%20-%204181&rft.eissn=0003-2700&rft.issn=0003-2700&rft.au=GIRAULT,%20Mathias&BENEYTON,%20Thomas&PEKIN,%20Deniz&BUISSON,%20Lionel&BICHON,%20Sabrina&rft.genre=article |
