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Modelling metabolic fluxes of tomato stems reveals that nitrogen shapes central metabolism for defence against Botrytis cinerea

hal.structure.identifierPlantes et systèmes de culture horticoles [PSH]
hal.structure.identifierDémarche intégrée pour l'obtention d'aliments de qualité [UMR QualiSud]
dc.contributor.authorLACRAMPE, Nathalie
hal.structure.identifierPlantes et systèmes de culture horticoles [PSH]
dc.contributor.authorLUGAN, Raphaël
hal.structure.identifierPlantes et systèmes de culture horticoles [PSH]
dc.contributor.authorDUMONT, Doriane
hal.structure.identifierUnité de Pathologie Végétale [PV]
dc.contributor.authorNICOT, Philippe C.
hal.structure.identifierPlantes et systèmes de culture horticoles [PSH]
dc.contributor.authorLECOMPTE, François
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCOLOMBIE, Sophie
dc.date.issued2024-03-29
dc.identifier.issn0022-0957
dc.description.abstractEnAmong plant pathogens, the necrotrophic fungus Botrytis cinerea is one of the most prevalent, leading to severe crop damage. Studies related to its colonization of different plant species have reported variable host metabolic responses to infection. In tomato, high N availability leads to decreased susceptibility. Metabolic flux analysis can be used as an integrated method to better understand which metabolic adaptations lead to effective host defence and resistance. Here, we investigated the metabolic response of tomato infected by B. cinerea in symptomless stem tissues proximal to the lesions, with a reconstructed metabolic model constrained with a large and consistent metabolic dataset acquired under four different N supplies, throughout 7 days post inoculation (dpi). An overall comparison of 48 flux solution vectors of Botrytis- and mock-inoculated plants showed that fluxes were higher in Botrytis-inoculated plants, and the difference increased with a reduction in available N, accompanying an unexpected increase in radial growth. Despite higher fluxes, such as those involved in cell wall synthesis and other pathways, fluxes related to glycolysis, the TCA cycle, amino acids and protein synthesis were limited under very low N, which might explain the enhanced susceptibility. Limiting starch synthesis and enhancing fluxes towards redox and specialized metabolism also contributed to defence independent of N supply.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subject.enTomato (Solanum lycopersicum)
dc.subject.enNitrogen
dc.subject.enBotrytis cinerea
dc.subject.enMetabolic fluxes
dc.subject.enConstraint-based modelling
dc.subject.enCentral metabolism
dc.subject.enNecrotrophic pathogens
dc.title.enModelling metabolic fluxes of tomato stems reveals that nitrogen shapes central metabolism for defence against Botrytis cinerea
dc.typeArticle de revue
dc.identifier.doi10.1093/jxb/erae140
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologie/Physiologie [q-bio.TO]
bordeaux.journalJournal of Experimental Botany
bordeaux.pageerae140
bordeaux.peerReviewedoui
hal.identifierhal-04554488
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04554488v1
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