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Effects of Methylmercury Contained in a Diet Mimicking the Wayana Amerindians Contamination through Fish Consumption: Mercury Accumulation, Metallothionein Induction, Gene Expression Variations, and Role of the Chemokine CCL2

dc.rights.licenseopenen_US
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOURDINEAUD, Jean-Paul
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorLACLAU, Muriel
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorMAURY-BRACHET, Régine
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBAUDRIMONT, Magalie
IDREF: 105696722
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorMESMER-DUDONS, Nathalie
dc.contributor.authorFUJIMURA, Masatake
dc.contributor.authorMARIGHETTO, Aline
dc.contributor.authorGODEFROY, David
dc.contributor.authorROSTÈNE, William
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorBRÈTHES, Daniel
dc.date.accessioned2024-04-16T09:08:58Z
dc.date.available2024-04-16T09:08:58Z
dc.date.issued2012-06-21
dc.identifier.issn1422-0067en_US
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/22837723
dc.identifier.urihttps://hal.archives-ouvertes.fr/hal-00749442
dc.identifier.urioai:crossref.org:10.3390/ijms13067710
dc.identifier.urioadoi:http://europepmc.org/articles/pmc3397555?pdf=render
dc.identifier.uriftpubmed:oai:pubmedcentral.nih.gov:3397555
dc.identifier.uriftunivpmcurie:oai:HAL:hal-00749442v1
dc.identifier.urioai:researchgate.net:230574289
dc.identifier.urioadoi:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397555
dc.identifier.urioadoi:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397555/pdf
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199080
dc.description.abstractEnMethylmercury (MeHg) is a potent neurotoxin, and human beings are mainly exposed to this pollutant through fish consumption. We addressed the question of whether a diet mimicking the fish consumption of Wayanas Amerindians from French Guiana could result in observable adverse effects in mice. Wayanas adult men are subjected to a mean mercurial dose of 7 g Hg/week/kg of body weight. We decided to supplement a vegetarian-based mice diet with 0.1% of lyophilized Hoplias aimara fish, which Wayanas are fond of and equivalent to the same dose as that afflicting the Wayanas Amerindians. Total mercury contents were 1.4 ± 0.2 and 5.4 ± 0.5 ng Hg/g of food pellets for the control and aimara diets, respectively. After 14 months of exposure, the body parts and tissues displaying the highest mercury concentration on a dry weight (dw) basis were hair (733 ng/g) and kidney (511 ng/g), followed by the liver (77 ng/g). Surprisingly, despite the fact that MeHg is a neurotoxic compound, the brain accumulated low levels of mercury (35 ng/g in the cortex). The metallothionein (MT) protein concentration only increased in those tissues (kidney, muscles) in which MeHg demethylation had occurred. This can be taken as a molecular sign of divalent mercurial contamination since only Hg2+ has been reported yet to induce MT accumulation in contaminated tissues. The suppression of the synthesis of the chemokine CCL2 in the corresponding knockout (KO) mice resulted in important changes in gene expression patterns in the liver and brain. After three months of exposure to an aimara-containing diet, eight of 10 genes selected (Sdhb, Cytb, Cox1, Sod1, Sod2, Mt2, Mdr1a and Bax) were repressed in wild-type mice liver whereas none presented a differential expression in KO Ccl2−/− mice. In the wild-type mice brain, six of 12 genes selected (Cytb, Cox1, Sod1, Sod2, Mdr1a and Bax) presented a stimulated expression, whereas all remained at the basal level of expression in KO Ccl2−/− mice. In the liver of aimara-fed mice, histological alterations were observed for an accumulated mercury concentration as low as 32 ng/g, dw, and metal deposits were observed within the cytoplasm of hepatic cells.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.sourcecrossref
dc.sourceoadoi_repo
dc.sourcebase
dc.sourceresearchgate
dc.title.enEffects of Methylmercury Contained in a Diet Mimicking the Wayana Amerindians Contamination through Fish Consumption: Mercury Accumulation, Metallothionein Induction, Gene Expression Variations, and Role of the Chemokine CCL2
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/ijms13067710en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalInternational Journal of Molecular Sciencesen_US
bordeaux.page7710-7738en_US
bordeaux.volume13en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue6en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEAen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=International%20Journal%20of%20Molecular%20Sciences&rft.date=2012-06-21&rft.volume=13&rft.issue=6&rft.spage=7710-7738&rft.epage=7710-7738&rft.eissn=1422-0067&rft.issn=1422-0067&rft.au=BOURDINEAUD,%20Jean-Paul&LACLAU,%20Muriel&MAURY-BRACHET,%20R%C3%A9gine&GONZALEZ,%20Patrice&BAUDRIMONT,%20Magalie&rft.genre=article


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