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Effects of dietary methylmercury on the zebrafish brain: Histological, mitochondrial, and gene transcription analyses

dc.rights.licenseopenen_US
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorCAMBIER, Sébastien
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorMESMER-DUDONS, Nathalie
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorBRÈTHES, Daniel
dc.contributor.authorFUJIMURA, Masatake
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOURDINEAUD, Jean-Paul
dc.date.accessioned2024-04-16T08:47:53Z
dc.date.available2024-04-16T08:47:53Z
dc.date.issued2011-09-25
dc.identifier.issn0966-0844en_US
dc.identifier.urihttps://www.researchgate.net/publication/51674566_Effects_of_dietary_methylmercury_on_the_zebrafish_brain_Histological_mitochondrial_and_gene_transcription_analyses
dc.identifier.urioai:crossref.org:10.1007/s10534-011-9494-6
dc.identifier.urioai:researchgate.net:51674566
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199075
dc.description.abstractEnThe neurotoxic compound methylmercury (MeHg) is a commonly encountered pollutant in the environment, and constitutes a hazard for wildlife and human health through fish consumption. To study the neurotoxic impact of MeHg on piscivorous fish, we contaminated the model fish species Danio rerio for 25 and 50 days with food containing 13.5 μg/g dry weight (dw) of MeHg (0.6 μg MeHg/fish/day), an environmentally relevant dose leading to brain mercury concentrations of 30 ± 4 μg of Hg g(-1) (dw) after 25 days of exposure and 46 ± 7 μg of Hg g(-1) (dw) after 50 days. Brain mitochondrial respiration was not modified by exposure to MeHg, contrary to what happens in skeletal muscles. A 6-fold increase in the expression of the sdh gene encoding the succinate dehydrogenase Fe/S protein subunit was detected in the contaminated brain after 50 days of exposure. An up regulation of 3 genes, atp2b3a, atp2b3b, and slc8a2b, encoding for calcium transporters was noticed after 25 days of exposure but the atp2b3a and atp2b3b were repressed and the slc8a2b gene expression returned to its basal level after 50 days, suggesting a perturbation of calcium homeostasis. After 50 days, we detected the up regulation of glial fibrillary acidic protein and glutathione S-transferase genes (gfap and gst), along with a repression of the glutathione peroxidase gene gpx1. These results match well with a MeHg-induced onset of oxidative stress and inflammation. A transmission electron microscopic observation confirmed an impairment of the optical tectum integrity, with a decrease of the nucleal area in contaminated granular cells compared to control cells, and a lower density of cells in the contaminated tissue. A potential functional significance of such changes observed in optical tectum when considering wild fish contaminated in their natural habitat might be an impaired vision and therefore a lowered adaptability to their environment.
dc.language.isoENen_US
dc.sourcecrossref
dc.sourceresearchgate
dc.title.enEffects of dietary methylmercury on the zebrafish brain: Histological, mitochondrial, and gene transcription analyses
dc.typeArticle de revueen_US
dc.identifier.doi10.1007/s10534-011-9494-6en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalBioMetalsen_US
bordeaux.page165-180en_US
bordeaux.volume25en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue1en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEAen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-04547964
hal.version1
hal.date.transferred2024-04-16T08:47:55Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=BioMetals&rft.date=2011-09-25&rft.volume=25&rft.issue=1&rft.spage=165-180&rft.epage=165-180&rft.eissn=0966-0844&rft.issn=0966-0844&rft.au=CAMBIER,%20S%C3%A9bastien&GONZALEZ,%20Patrice&MESMER-DUDONS,%20Nathalie&BR%C3%88THES,%20Daniel&FUJIMURA,%20Masatake&rft.genre=article


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