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dc.rights.licenseopenen_US
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorCAMBIER, Sébastien
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorDURRIEU, Gilles
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOURDINEAUD, Jean-Paul
dc.date.accessioned2024-04-16T08:02:48Z
dc.date.available2024-04-16T08:02:48Z
dc.date.issued2009-11-01
dc.identifier.issn0147-6513en_US
dc.identifier.urihttps://www.researchgate.net/publication/40035443_Cadmium-Induced_genotoxicity_in_zebrafish_at_environmentally_relevant_doses
dc.identifier.urioai:researchgate.net:40035443
dc.identifier.urioai:crossref.org:10.1016/j.ecoenv.2009.10.012
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199069
dc.description.abstractEnGenotoxic effects of cadmium on zebra fish Danio rerio have been assessed by random amplified polymorphic DNA and real time PCR, followed by a comparison of the melting temperature patterns between each amplification reaction. Fish were exposed to two concentrations of cadmium chloride dissolved in the medium (1.9+/-0.6 microg Cdl(-1), C(1); 9.6+/-2.9 microg Cdl(-1), C(2)) for 21 days. A discriminative RAPD probe, OPB11, was first selected producing differential band patterns between control and metal-exposed genomic DNAs. RAPD-PCR showed an increase in the relative hybridization efficiency of OPB11 on the genomic DNAs coming from fish exposed to both Cd concentrations as compared to the control condition. In addition, the RAPD-PCR melting temperature patterns showed that with the OPB11 probe, the frequency of PCR products whose fusion temperature belongs to the [86-87 degrees C] interval decreased with Cd contamination, whereas an increase of frequency for the [78-80 degrees C] and [85-86 degrees C] intervals was correlated with Cd exposure.
dc.language.isoENen_US
dc.sourceresearchgate
dc.sourcecrossref
dc.title.enCadmium-Induced genotoxicity in zebrafish at environmentally relevant doses
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.ecoenv.2009.10.012en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalEcotoxicology and Environmental Safetyen_US
bordeaux.page312-319en_US
bordeaux.volume73en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue3en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEAen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-04547838
hal.version1
hal.date.transferred2024-04-16T08:02:51Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Ecotoxicology%20and%20Environmental%20Safety&rft.date=2009-11-01&rft.volume=73&rft.issue=3&rft.spage=312-319&rft.epage=312-319&rft.eissn=0147-6513&rft.issn=0147-6513&rft.au=CAMBIER,%20S%C3%A9bastien&GONZALEZ,%20Patrice&DURRIEU,%20Gilles&BOURDINEAUD,%20Jean-Paul&rft.genre=article


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