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Comparative analysis of gene expression in brain, liver, skeletal muscles, and gills of zebrafish (Danio rerio) exposed to environmentally relevant waterborne uranium concentrations

dc.rights.licenseopenen_US
dc.contributor.authorLEREBOURS, Adelaide
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
dc.contributor.authorADAM, Christelle
dc.contributor.authorCAMILLERI, Virginie
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOURDINEAUD, Jean-Paul
dc.contributor.authorGARNIER-LAPLACE, Jacqueline
dc.date.accessioned2024-04-16T07:51:47Z
dc.date.available2024-04-16T07:51:47Z
dc.date.issued2009-06-01
dc.identifier.urihttps://www.researchgate.net/publication/23790288_Comparative_analysis_of_gene_expression_in_brain_liver_skeletal_muscles_and_gills_of_zebrafish_Danio_rerio_exposed_to_environmentally_relevant_waterborne_uranium_concentrations
dc.identifier.uriftunivportsmpubl:oai:researchportal.port.ac.uk:publications/201e012d-54de-4d53-9dda-2a4ae06ab362
dc.identifier.urioai:researchgate.net:23790288
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199065
dc.description.abstractEnThe effects of waterborne uranium (U) exposure on gene expression were examined in four organs (brain, liver, skeletal muscles, and gills) of the zebrafish (Danio rerio). Adult male fish were exposed to three treatments: No added uranium (control), 23 ± 6 μg U/L, and 130 ± 34 μg U/L. After 3, 10, 21, and 28 d of exposure and an 8-d depuration period, gene expression and uranium bioaccumulation were analyzed. Bioaccumulation decreased significantly in liver during the depuration phase, and genes involved in detoxification, apoptotic mechanism, and immune response were strongly induced. Among these genes, abcb3l1, which belongs to the adenosine triphosphate (ATP)—binding cassette transporter family, was induced 4- and 24-fold in organisms previously exposed to 23 ± 6 and 130 ± 34 μg U/L, respectively. These results highlight the role of liver in detoxification mechanisms. In gills, at the highest uranium concentration, gpx1a, cat, sod1, and sod2 genes were up-regulated at day 21, indicating the onset of an oxidative stress. Mitochondrial metabolism and DNA integrity also were affected, because coxI, atp5f1, and rad51 genes were up-regulated at day 21 and during the depuration phase. In skeletal muscles, coxI, atp5f1, and cat were induced at day 3, suggesting an impact on the mitochondrial metabolism and production of reactive oxygen species. In brain, glsI also was induced at day 3, suggesting a need in the glutamate synthesis involved with neuron transmission. No changes in gene expression were observed in brain and skeletal muscles at days 21 and 28, although bioaccumulation increased. During the depuration phase, uranium excretion was inefficient in brain and skeletal muscles, and expression of most of the tissue-specific genes was repressed or unchanged.
dc.language.isoENen_US
dc.sourcecrossref
dc.sourceresearchgate
dc.title.enComparative analysis of gene expression in brain, liver, skeletal muscles, and gills of zebrafish (Danio rerio) exposed to environmentally relevant waterborne uranium concentrations
dc.typeArticle de revueen_US
dc.identifier.doi10.1897/08-357.1en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalEnvironmental Toxicology and Chemistryen_US
bordeaux.page1271-1278en_US
bordeaux.volume28en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue6en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEAen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
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