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At environmental doses, dietary methylmercury inhibits mitochondrial energy metabolism in skeletal muscles of the zebra fish (Danio rerio).

dc.rights.licenseopenen_US
dc.contributor.authorCAMBIER, S.
dc.contributor.authorBÉNARD, G.
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorMESMER-DUDONS, Nathalie
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
hal.structure.identifierLaboratoire Maladies Rares: Génétique et Métabolisme (Bordeaux) [U1211 INSERM/MRGM]
dc.contributor.authorROSSIGNOL, Rodrigue
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorBRÈTHES, D.
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOURDINEAUD, Jean-Paul
dc.date.accessioned2024-04-15T16:21:02Z
dc.date.available2024-04-15T16:21:02Z
dc.date.issued2009-01-01
dc.identifier.issn1357-2725en_US
dc.identifier.urihttp://hal.archives-ouvertes.fr/hal-00374017/en/
dc.identifier.urioai:crossref.org:10.1016/j.biocel.2008.08.008
dc.identifier.uriftccsdartic:oai:hal.archives-ouvertes.fr:hal-00374017
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199019
dc.description.abstractEnThe neurotoxic compound methylmercury (MeHg) is a commonly encountered pollutant in the environment, and constitutes a hazard for human health through fish eating. To study the impact of MeHg on mitochondrial structure and function, we contaminated the model fish species Danio rerio with food containing 13 μg of MeHg per gram, an environmentally relevant dose. Mitochondria from contaminated zebrafish muscles presented structural abnormalities under electron microscopy observation. In permeabilized muscle fibers, we observed, a strong inhibition of both state 3 mitochondrial respiration and functionally isolated maximal cytochrome c oxidase (COX) activity after 49 days of MeHg exposure. However, the state 4 respiratory rate remained essentially unchanged. This suggested a defect at the level of ATP synthesis. Accordingly, we measured a dramatic decrease in the rate of ATP release by skinned muscle fibers using either pyruvate and malate or succinate as respiratory substrates. However, the amount and the assembly of the ATP synthase were identical in both control and contaminated muscle mitochondrial fractions. This suggests that MeHg induced a decoupling of mitochondrial oxidative phosphorylation in the skeletal muscle of zebrafish. Western blot analysis showed a 30% decrease of COX subunit IV levels, a 50% increase of ATP synthase subunit α, and a 40% increase of the succinate dehydrogenase Fe/S protein subunit in the contaminated muscles. This was confirmed by the analysis of gene expression levels, using RT-PCR. Our study provides a basis for further analysis of the deleterious effect of MeHg on fish health via mitochondrial impairment.
dc.language.isoENen_US
dc.sourcecrossref
dc.sourcebase
dc.title.enAt environmental doses, dietary methylmercury inhibits mitochondrial energy metabolism in skeletal muscles of the zebra fish (Danio rerio).
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.biocel.2008.08.008en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalInternational Journal of Biochemistry and Cell Biologyen_US
bordeaux.page791-799en_US
bordeaux.volume41en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue4en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEAen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-04547489
hal.version1
hal.date.transferred2024-04-15T16:21:04Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=International%20Journal%20of%20Biochemistry%20and%20Cell%20Biology&rft.date=2009-01-01&rft.volume=41&rft.issue=4&rft.spage=791-799&rft.epage=791-799&rft.eissn=1357-2725&rft.issn=1357-2725&rft.au=CAMBIER,%20S.&B%C3%89NARD,%20G.&MESMER-DUDONS,%20Nathalie&GONZALEZ,%20Patrice&ROSSIGNOL,%20Rodrigue&rft.genre=article


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