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dc.rights.licenseopenen_US
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorMARIE, Véronique
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBAUDRIMONT, Magalie
IDREF: 105696722
dc.contributor.authorBOUTET, Isabelle
dc.contributor.authorMORAGA, Dario
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOURDINEAUD, Jean-Paul
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOUDOU, Alain
dc.date.accessioned2024-04-15T14:23:25Z
dc.date.available2024-04-15T14:23:25Z
dc.date.issued2006-01-01
dc.identifier.issn0730-7268en_US
dc.identifier.urihttp://hal.univ-brest.fr/hal-00663795
dc.identifier.urioai:crossref.org:10.1897/05-114r.1
dc.identifier.uriftunivbrest:oai:HAL:hal-00663795v1
dc.identifier.urioai:researchgate.net:7259410
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199010
dc.description.abstractEnQuantitative real-time polymerase chain reaction (PCR) was used to compare for the first time the differential expression of metallothionein (MT) isoform genes, together with biosynthesis of the total MT proteins, in the gills of triploid and diploid juvenile Pacific oyster Crassostrea gigas in response to cadmium (Cd) and zinc (Zn) exposure. Oysters were exposed to Cd (0.133 mM), Zn (15.3 mM), and Cd1Zn for 14 d. Results showed similar response capacities to metal exposures in the two populations. No significant difference was revealed in terms of MT gene expression, MT protein synthesis, and Cd accumulation. However, triploid oysters bioaccumulated Zn 30% less efficiently than diploid oysters. Among the three MT isoform genes, CgMT2 appeared to be more expressed than CgMT1, whereas CgMT3 appeared to be anecdotal (106 times lower than CgMT2). CgMT2 and CgMT1 gene expression levels were increased sevenfold in the presence of Cd, whereas Zn appeared to have no effect. A twofold increase in MT protein levels occurred in response to Cd exposure. Discrepancies between mRNA and protein levels suggest that in C. gigas MT are regulated at the transcriptional level, as well as at the translational level.
dc.language.isoENen_US
dc.sourcecrossref
dc.sourcebase
dc.sourceresearchgate
dc.title.enMetallothionein gene expression and protein levels in triploid and diploid oysters Crassostrea gigas after exposure to cadmium and zinc.
dc.typeArticle de revueen_US
dc.identifier.doi10.1897/05-114r.1en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalEnvironmental Toxicology and Chemistryen_US
bordeaux.page412en_US
bordeaux.volume25en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue2en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEAen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Environmental%20Toxicology%20and%20Chemistry&rft.date=2006-01-01&rft.volume=25&rft.issue=2&rft.spage=412&rft.epage=412&rft.eissn=0730-7268&rft.issn=0730-7268&rft.au=MARIE,%20V%C3%A9ronique&GONZALEZ,%20Patrice&BAUDRIMONT,%20Magalie&BOUTET,%20Isabelle&MORAGA,%20Dario&rft.genre=article


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