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An innovative and integrative assay for toxicity testing using individual fish embryos. Application to oxazepam

dc.rights.licenseopenen_US
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGRANGER JOLY DE BOISSEL, Philippine
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
dc.contributor.authorBULETÉ, Audrey
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorDAFFE, Guillemine
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorCLERANDEAU, Christelle
dc.contributor.authorVULLIET, Emmanuelle
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorCACHOT, Jérôme
ORCID: 0000-0002-1492-9684
IDREF: 19667672X
dc.date.accessioned2024-04-15T12:38:03Z
dc.date.available2024-04-15T12:38:03Z
dc.date.issued2017-08-01
dc.identifier.issn0045-6535en_US
dc.identifier.urioai:crossref.org:10.1016/j.chemosphere.2017.04.067
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199005
dc.description.abstractEnThis paper describes the development of an integrative embryo-toxicity assay in Japanese medaka allowing analysis of several toxicological endpoints together in a same individual. In this assay, embryos are topically exposed, and survival, hatching success, malformations, biometry, behaviour, and target gene expression are subsequently analysed in each individual. This assay was applied to oxazepam, an anxiolytic pharmaceutical compound currently found in wastewater treatment plant effluent. Even if oxazepam accumulation in embryos was very low, it caused spinal and cardiac malformations, delayed growth, erratic swimming and deregulation of genes involved in apoptosis, DNA repair and mitochondrial metabolism. Relationship between gene deregulation, abnormal behaviour, and developmental anomalies was demonstrated. This assay is sensitive enough to detect adverse effects at low chemical concentrations and at multiple endpoints in a unique fish embryo. This integrative embryo-toxicity assay is a powerful tool to characterize the spectrum of effects of new chemicals and also to link effects induced at different molecular, tissue and physiological levels.
dc.language.isoENen_US
dc.sourcecrossref
dc.subject.enAnxiolytic pharmaceutical
dc.subject.enBehaviour
dc.subject.enFish embryo-toxicity assay
dc.subject.enGene expression
dc.subject.enIndividual analyses
dc.subject.enMalformations
dc.title.enAn innovative and integrative assay for toxicity testing using individual fish embryos. Application to oxazepam
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.chemosphere.2017.04.067en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalChemosphereen_US
bordeaux.page468-477en_US
bordeaux.volume181en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEA
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Chemosphere&rft.date=2017-08-01&rft.volume=181&rft.spage=468-477&rft.epage=468-477&rft.eissn=0045-6535&rft.issn=0045-6535&rft.au=GRANGER%20JOLY%20DE%20BOISSEL,%20Philippine&GONZALEZ,%20Patrice&BULET%C3%89,%20Audrey&DAFFE,%20Guillemine&CLERANDEAU,%20Christelle&rft.genre=article


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