A glyphosate-based herbicide induces sub-lethal effects in early life stages and liver cell line of rainbow trout, Oncorhynchus mykiss
| dc.rights.license | open | en_US |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | WEEKS SANTOS, Shannon | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | GONZALEZ, Patrice
ORCID: 0000-0003-2628-2993 IDREF: 14341917X | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | CORMIER, Bettie | |
| dc.contributor.author | MAZZELLA, Nicolas | |
| dc.contributor.author | BONNAUD, Bertille | |
| dc.contributor.author | MORIN, Soizic | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | CLERANDEAU, Christelle | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | MORIN, Benedicte
IDREF: 178000892 | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | CACHOT, Jérôme
ORCID: 0000-0002-1492-9684 IDREF: 19667672X | |
| dc.date.accessioned | 2024-04-15T12:01:49Z | |
| dc.date.available | 2024-04-15T12:01:49Z | |
| dc.date.issued | 2019-11-01 | |
| dc.identifier.issn | 0166-445X | en_US |
| dc.identifier.uri | oai:crossref.org:10.1016/j.aquatox.2019.105291 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/199000 | |
| dc.description.abstractEn | Most pesticides used in agriculture end up in the aquatic environment through runoff and leaching of treated crops. One of the most commonly used herbicides is glyphosate. This compound or its metabolites are frequently detected in surface water in Europe. In the present study, in vivo and in vitro studies were carried out using the early life stages of rainbow trout (Oncorhynchus mykiss) and the cell line RTL-W1 (a liver cell line from rainbow trout) to characterize the toxic effects of glyphosate at environmentally-realistic concentrations. Both studies were performed using the commercial formulation Roundup® GT Max, and technical-grade glyphosate for the in vitro study. Eyed-stage embryos were exposed for 3 weeks to sub-lethal concentrations (0.1 and 1 mg/L) of glyphosate using Roundup. Numerous toxicity endpoints were recorded such as survival, hatching success, larval biometry, developmental abnormalities, swimming activity, genotoxicity (formamidopyrimidine DNA-glycosylase Fpg-modified comet assay), lipid peroxidation (TBARS), protein carbonyls and target gene transcription. Concentrations neither affected embryonic or larval survival nor increased developmental abnormalities. However, a significant decrease was observed in the head size of larvae exposed to 1 mg/L of glyphosate. In addition, a significant increase in mobility was observed for larvae exposed to glyphosate at 0.1 mg/L. TBARS levels were significantly decreased on larvae exposed to 1 mg/L (a.i.), and cat and cox1 genes were differently transcribed from controls. DNA damage was detected by the Fpg-modified comet assay in RTL-W1 cell line exposed to the technical-grade glyphosate and Roundup formulation. The results suggest that chronic exposure to glyphosate, at environmental concentrations, could represent a potential risk for early life stages of fish. | |
| dc.language.iso | EN | en_US |
| dc.source | crossref | |
| dc.subject.en | pesticide | |
| dc.subject.en | fish embryos | |
| dc.subject.en | liver cell line | |
| dc.subject.en | cytotoxicity | |
| dc.subject.en | embryotoxicity | |
| dc.subject.en | teratogenicity | |
| dc.subject.en | genotoxicity | |
| dc.subject.en | photomotor response | |
| dc.title.en | A glyphosate-based herbicide induces sub-lethal effects in early life stages and liver cell line of rainbow trout, Oncorhynchus mykiss | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.aquatox.2019.105291 | en_US |
| dc.subject.hal | Sciences de l'environnement | en_US |
| bordeaux.journal | Aquatic Toxicology | en_US |
| bordeaux.page | 105291 | en_US |
| bordeaux.volume | 216 | en_US |
| bordeaux.hal.laboratories | EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.team | EA | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | dissemin | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | dissemin | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Aquatic%20Toxicology&rft.date=2019-11-01&rft.volume=216&rft.spage=105291&rft.epage=105291&rft.eissn=0166-445X&rft.issn=0166-445X&rft.au=WEEKS%20SANTOS,%20Shannon&GONZALEZ,%20Patrice&CORMIER,%20Bettie&MAZZELLA,%20Nicolas&BONNAUD,%20Bertille&rft.genre=article |
