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Combination of photodynamic therapy and gene silencing achieved through the hierarchical self-assembly of porphyrin-siRNA complexes

dc.rights.licenseopen
dc.contributor.authorLAROUI, Nabila
dc.contributor.authorCOSTE, Maëva
hal.structure.identifierInstitut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
dc.contributor.authorLICHON, Laure
hal.structure.identifierCentre de Biochimie Structurale [Montpellier] [CBS]
dc.contributor.authorBESSIN, Yannick
hal.structure.identifierInstitut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
dc.contributor.authorGARY-BOBO, Magali
hal.structure.identifierLaboratoire de chimie de coordination [LCC]
dc.contributor.authorPRATVIEL, Geneviève
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorBONDUELLE, Colin
IDREF: 134527046
hal.structure.identifierCentre de recherches de biochimie macromoléculaire [CRBM]
dc.contributor.authorBETTACHE, Nadir
dc.contributor.authorULRICH, Sébastien
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2019
dc.identifier.issn0378-5173
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/19831
dc.description.abstractEnIn this work, we implemented a supramolecular approach in order to combine photodynamic therapy (PDT) with gene therapy. We made use of a simple cationic guanidylated porphyrin (H2‑PG) with the hypothesis that porphyrin aggregates should be capable of complexing siRNA through multivalent interactions and thus contribute to its intracellular delivery, while remaining active photosensitizers for PDT. The PDT effect of H2‑PG was shown by incubating human breast cancer cells (MDA-MB-231) with H2‑PG followed by light-irradiation at 405 nm. On the other hand, while siRNA do not enter cells alone, we showed, by fluorescence confocal microscopy and flow cytometry, that H2‑PG promotes the internalization of Atto-488 siRNA. Finally, studying the combined PDT and delivery of siRNA directed against inhibitory apoptotic protein (IAP) family, we found an additive effect of the two therapies, thereby demonstrating that H2‑PG is capable of acting both as a photosensitizer and supramolecular siRNA vector.
dc.language.isoen
dc.publisherElsevier
dc.subject.enCombination therapy
dc.subject.enPhotodynamic therapy
dc.subject.enGene therapy
dc.subject.enSupramolecular self-assembly
dc.subject.enPorphyrin
dc.subject.ensiRNA
dc.title.enCombination of photodynamic therapy and gene silencing achieved through the hierarchical self-assembly of porphyrin-siRNA complexes
dc.typeArticle de revue
dc.identifier.doi10.1016/j.ijpharm.2019.118585
dc.subject.halChimie
bordeaux.journalInternational Journal of Pharmaceutics
bordeaux.page118585
bordeaux.volume569
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-02322136
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02322136v1
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