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Nucleic acids complexation with cationic elastin-like polypeptides: Stoichiometry and stability of nano-assemblies

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hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorBRAVO-ANAYA, Lourdes Monica
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorGARBAY, Bertrand
IDREF: 033777551
hal.structure.identifierCentro Universitario UTEG [UTEG]
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorNANDO-RODRÍGUEZ, Jesrael L.E.
hal.structure.identifierCentro Universitario UTEG [UTEG]
dc.contributor.authorCARVAJAL RAMOS, Francisco
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorIBARBOURE, Emmanuel
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBATHANY, Katell
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorXIA, Yunjie
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorROSSELGONG, Julien
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorJOUCLA, Gilles
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorGARANGER, Elisabeth
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLECOMMANDOUX, Sebastien
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2019
dc.identifier.issn0021-9797
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/19780
dc.description.abstractEnPositively charged elastin-like polypeptides (ELPs) were synthesized for the compaction of genetic material. A recombinant ELP (VPGXG)40 with X = V,M (3:1) was post-modified in two steps to introduce chemoselectively either primary or secondary amine pendant groups at each methionine residue. Positively charged ELPs were characterized by SDS-PAGE, size exclusion chromatography, 1H NMR, potentiometric titrations and dynamic light scattering to assess their purity and determine their degree of functionalization, molecular weight, isoelectric point and thermo-responsive behaviour. Electrostatic complexation between the different ELP derivatives and nucleic acids was studied to determine the stoichiometry of ELPS/nucleic acids complex formation, and to find optimal conditions leading to stable nanoparticles with controlled size and surface potential. The stability of these complexes was investigated in the presence of salts at physiological concentrations and in the presence of surfactant. This study revealed that two regimes of stable nanoparticles in terms of size and charge can be obtained from the electrostatic complexation between the primary amine containing ELP derivative, ELP(-NH2), and plasmid DNA. Resulting complexes were found to be stable to dissociation for charge ratios up to 2.5 under physiological salt concentrations (154 mM NaCl), showing that plasmid DNA was completely condensed by the polycationic ELP and protected against electrolyte-mediated dissociation.
dc.description.sponsorshipDéveloppement de squelettes polypeptidiques recombinants pour la synthèse de glycoconjugués multivalents parfaitement définis - ANR-15-CE07-0002
dc.language.isoen
dc.publisherElsevier
dc.subject.enPolyelectrolyte complexes
dc.subject.enDNA complexation
dc.subject.enElastin-like polypeptides
dc.subject.enChemoselective thioalkylation
dc.title.enNucleic acids complexation with cationic elastin-like polypeptides: Stoichiometry and stability of nano-assemblies
dc.typeArticle de revue
dc.identifier.doi10.1016/j.jcis.2019.09.054
dc.subject.halPhysique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
dc.subject.halChimie/Polymères
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
bordeaux.journalJournal of Colloid and Interface Science
bordeaux.page777-792
bordeaux.volume557
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-02378593
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02378593v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Colloid%20and%20Interface%20Science&rft.date=2019&rft.volume=557&rft.spage=777-792&rft.epage=777-792&rft.eissn=0021-9797&rft.issn=0021-9797&rft.au=BRAVO-ANAYA,%20Lourdes%20Monica&GARBAY,%20Bertrand&NANDO-RODR%C3%8DGUEZ,%20Jesrael%20L.E.&CARVAJAL%20RAMOS,%20Francisco&IBARBOURE,%20Emmanuel&rft.genre=article


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