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Role of metallothioneins in superoxide radical generation during copper redox cycling: Defining the fundamental function of metallothioneins

dc.rights.licenseopenen_US
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorACHARD-JORIS, Maud
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorMOREAU, Jean-Luc
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorLUCAS, Megumi
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBAUDRIMONT, Magalie
IDREF: 105696722
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorMESMER-DUDONS, Nathalie
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOUDOU, Alain
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOURDINEAUD, Jean-Paul
dc.date.accessioned2024-04-15T08:56:06Z
dc.date.available2024-04-15T08:56:06Z
dc.date.issued2007-12-01
dc.identifier.issn0300-9084en_US
dc.identifier.urihttps://www.researchgate.net/publication/6157180_Role_of_metallothioneins_in_superoxide_radical_generation_during_copper_redox_cycling_Defining_the_fundamental_function_of_metallothioneins
dc.identifier.urioai:crossref.org:10.1016/j.biochi.2007.06.005
dc.identifier.urioai:researchgate.net:6157180
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197584
dc.description.abstractEnIn order to demonstrate the in vivo antioxidant properties of metallothioneins (MTs), the bacteria Escherichia coli was used as a cell reactor in which we compared the metal binding and antioxidative functions of MTs from different species, with different structures and polypeptide lengths. No protective effects of cytoplasmic MTs from cadmium (Cd) or zinc (Zn) contamination were observed in a wild-type E. coli strain, although these MTs can efficiently bind both Cd and Zn. To test their antioxidant properties, MTs were expressed within the cytoplasm of a sodA sodB deficient mutated strain (QC1726). However, a paradoxical MT toxicity was found when this strain was contaminated with Cd and Zn, suggesting that in a wild-type strain, superoxide dismutase counteracts MT toxicity. The most toxic MT was the one with the strongest Cd and Zn binding capacities. This toxic effect was linked to the generation of superoxide radicals, since a Cd-contaminated QC1726 strain expressing oyster MT isoforms produced 75-85% more O(2)*(-) than the control QC1726 strain. Conversely, under anaerobiosis or in the presence of a copper chelator, MTs protected QC1726 strain from Cd and Zn contamination. A model is proposed to explain the observed MT toxicity.
dc.language.isoENen_US
dc.sourcecrossref
dc.sourceresearchgate
dc.title.enRole of metallothioneins in superoxide radical generation during copper redox cycling: Defining the fundamental function of metallothioneins
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.biochi.2007.06.005en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalBiochimieen_US
bordeaux.page1474-1488en_US
bordeaux.volume89en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue12en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEAen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-04546369
hal.version1
hal.date.transferred2024-04-15T08:56:08Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biochimie&rft.date=2007-12-01&rft.volume=89&rft.issue=12&rft.spage=1474-1488&rft.epage=1474-1488&rft.eissn=0300-9084&rft.issn=0300-9084&rft.au=ACHARD-JORIS,%20Maud&MOREAU,%20Jean-Luc&LUCAS,%20Megumi&BAUDRIMONT,%20Magalie&MESMER-DUDONS,%20Nathalie&rft.genre=article


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