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Molecular cloning, sequence and expression of Aa-polB, a mitochondrial gene encoding a family B DNA polymerase from the edible basidiomycete Agrocybe aegerita. Mol Gen Genet

dc.rights.licenseopenen_US
dc.contributor.authorBOIS, F.
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorBARROSO, Gerard
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
hal.structure.identifierUniversité de Bordeaux Ségalen [Bordeaux 2]
dc.contributor.authorLABARÈRE, Jacques
dc.date.accessioned2024-04-12T07:38:37Z
dc.date.available2024-04-12T07:38:37Z
dc.date.issued1999-04-01
dc.identifier.issn0026-8925en_US
dc.identifier.urihttps://www.researchgate.net/publication/12975919_Molecular_cloning_sequence_and_expression_of_Aa-polB_a_mitochondrial_gene_encoding_a_family_B_DNA_polymerase_from_the_edible_basidiomycete_Agrocybe_aegerita._Mol_Gen_Genet
dc.identifier.urioai:researchgate.net:12975919
dc.identifier.urioai:crossref.org:10.1007/s004380050994
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197569
dc.description.abstractEnAn ORF of 1716 nucleotides, putatively encoding a DNA polymerase, was characterized in the mitochondrial genome of the edible basidiomycete Agrocybe aegerita. The complete gene, named Aa-polB, and its flanking regions were cloned and sequenced from three overlapping restriction fragments. Aa-polB is located between the SSU rDNA (5' region) and a gene for tRNA(Asn) (3' region), and is separated from these genes by two A + T-rich intergenic regions of 1048 (5' region) and 3864 (3' region) nucleotides, which lack repeated sequences of mitochondrial or plasmid origin. The deduced Aa-POLB protein shows extensive sequence similarity with the family B DNA polymerases encoded by genomes that rely on protein-primed replication (invertrons). The domains involved in the 3'-->5' exonuclease (Exo I to III) and polymerase (Pol I to Pol V) activities were localized on the basis of conserved sequence motifs. The alignment of the Aa-POLB protein (571 amino acids) with sequences of family B DNA polymerases from invertrons revealed that in Aa-POLB the N-terminal region preceding Exo I is short, suggesting a close relationship with the DNA polymerases of bacteriophages that have linear DNA. The Aa-polB gene was shown to be present in all wild strains examined, which were collected from a wide range of locations in Europe. As shown by RT-PCR, the Aa-polB gene is transcribed in the mitochondria, at a low but significant level. The likelihood of the coexistence of Aa-POLB and Pol gamma in the A. aegerita mitochondrion is discussed in the light of recent reports showing the conservation of the nucleus-encoded Pol gamma from yeast to human.
dc.language.isoENen_US
dc.sourceresearchgate
dc.sourcecrossref
dc.title.enMolecular cloning, sequence and expression of Aa-polB, a mitochondrial gene encoding a family B DNA polymerase from the edible basidiomycete Agrocybe aegerita. Mol Gen Genet
dc.typeArticle de revueen_US
dc.identifier.doi10.1007/s004380050994en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalMolecular and General Genetics MGGen_US
bordeaux.page508-513en_US
bordeaux.volume261en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue3en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20and%20General%20Genetics%20MGG&rft.date=1999-04-01&rft.volume=261&rft.issue=3&rft.spage=508-513&rft.epage=508-513&rft.eissn=0026-8925&rft.issn=0026-8925&rft.au=BOIS,%20F.&BARROSO,%20Gerard&GONZALEZ,%20Patrice&LABAR%C3%88RE,%20Jacques&rft.genre=article


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