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A knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence dorée phytoplasma

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANUTO, Francesca
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDURET, Sybille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDUBRANA, Marie-Pierre
hal.structure.identifierPlateforme Protéome [Bordeaux]
dc.contributor.authorCLAVEROL, Stéphane
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMALEMBIC-MAHER, Sylvie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFOISSAC, Xavier
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorARRICAU-BOUVERY, Nathalie
dc.date.issued2023-11-06
dc.identifier.issn2235-2988
dc.description.abstractEnIntroduction The adhesion of flavescence dorée phytoplasma to the midgut epithelium cells of their insect vectors is partially mediated by the variable membrane protein A (VmpA), an adhesin which shows lectin properties. In order to identify the insect receptor for VmpA, we identified Euscelidius variegatus cell proteins interacting with recombinant VmpA-His 6 . Methods The E. variegatus proteins were identified by mass spectrometry analysis of VmpA- E. variegatus protein complexes formed upon in vitro interaction assays. To assess their impact in VmpA binding, we reduced the expression of the candidate genes on E. variegatus cells in culture by dsRNA-mediated RNAi. The effect of candidate gene knockdown on VmpA binding was measured by the capacity of E. variegatus cells to bind VmpA-coated fluorescent beads. Results and discussion There were 13 candidate proteins possessing potential N-glycosylation sites and predicted transmembrane domains selected. The decrease of expression of an unknown transmembrane protein with leucine-rich repeat domains (uk1_LRR) was correlated with the decreased adhesion of VmpA beads to E. variegatus cells. The uk1_LRR was more expressed in digestive tubes than salivary glands of E. variegatus . The protein uk1_LRR could be implicated in the binding with VmpA in the early stages of insect infection following phytoplasmas ingestion
dc.language.isoen
dc.publisherFrontiers
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enA knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence dorée phytoplasma
dc.typeArticle de revue
dc.identifier.doi10.3389/fcimb.2023.1289100
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalFrontiers in Cellular and Infection Microbiology
bordeaux.volume13
bordeaux.peerReviewedoui
hal.identifierhal-04506670
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04506670v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20Cellular%20and%20Infection%20Microbiology&rft.date=2023-11-06&rft.volume=13&rft.eissn=2235-2988&rft.issn=2235-2988&rft.au=CANUTO,%20Francesca&DURET,%20Sybille&DUBRANA,%20Marie-Pierre&CLAVEROL,%20St%C3%A9phane&MALEMBIC-MAHER,%20Sylvie&rft.genre=article


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