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The Zinc Finger protein Sl ZFP2 is essential for tomato fruit locular tissue morphogenesis

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorHOANG, Gabriel
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJOANA, Jorly
hal.structure.identifierPlantes et systèmes de culture horticoles [PSH]
dc.contributor.authorCONSTANTINESCU, Dario
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARTIN, Pascal
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGADIN, Stéphanie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUXION, Jean-Philippe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRÈS, Cécile
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGARCIA, Virginie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGONZALEZ, Nathalie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
hal.structure.identifierPlantes et systèmes de culture horticoles [PSH]
dc.contributor.authorBERTIN, Nadia
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFERNANDEZ-LOCHU, Lucie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLEMAIRE-CHAMLEY, Martine
dc.date.accessioned2024-04-11T10:05:41Z
dc.date.available2024-04-11T10:05:41Z
dc.date.issued2024-03-12
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197528
dc.description.abstractEnIn tomato ( Solanum lycopersicum L .) fruit, the locular tissue (LT) is a unique jelly-like tissue that differentiates from the central axis of the fruit after ovule fertilization. LT is essential for seed development and dispersal by preventing early germination and initiating fruit ripening. In this work, we studied a “ gel-less ” mutant and identified the underlying mutation in the coding sequence of the C2H2 zinc finger transcription factor (TF) Sl ZFP2. Histological, cytological and molecular characterization from knockout-CRISPR/Cas9 lines for this gene revealed the strong and early impact of zfp2 mutation on cell cycle and endocycle in LT. Additionally, model-based analysis of cellular data revealed that cell cycle was the main altered process, explaining the zfp2 mutant phenotype. Further laser capture microdissection coupled with RNA-Seq analysis of young LT highlighted global expression changes between WT and zfp2 mutant and led to a preliminary list of potential direct targets of the Sl ZFP2 TF. This multifaceted approach not only uncovered a new role for Sl ZFP2 TF as an essential regulator of LT morphogenesis, but also provides a foundation for future works aimed at deciphering the intricate regulatory networks governing fruit tissue development in tomato. One sentence summary Alteration of cell division and endoreduplication in a gel-less mutant reveals the role of the transcription factor Sl ZFP2 in tomato locular tissue morphogenesis
dc.language.isoen
dc.subject.enC2H2 zinc finger transcription factor ,SlZFP2, Tomato fruit development, Locular tissue morphogenesis
dc.title.enThe Zinc Finger protein Sl ZFP2 is essential for tomato fruit locular tissue morphogenesis
dc.typeArticle de revue
dc.typeArticle de synthèse
dc.identifier.doi10.1101/2024.03.07.582990
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalBioRxiv
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-04522689
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04522689v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=BioRxiv&rft.date=2024-03-12&rft.au=HOANG,%20Gabriel&JOANA,%20Jorly&CONSTANTINESCU,%20Dario&MARTIN,%20Pascal&GADIN,%20St%C3%A9phanie&rft.genre=article&unknown


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