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Comparison of the Molecular Responses of Tolerant, Susceptible and Highly Susceptible Grapevine Cultivars During Interaction With the Pathogenic Fungus Eutypa lata

hal.structure.identifierÉcologie et biologie des interactions [EBI [Poitiers]]
dc.contributor.authorCARDOT, Chloé
hal.structure.identifierÉcologie et biologie des interactions [EBI [Poitiers]]
dc.contributor.authorMAPPA, Gaëtan
hal.structure.identifierÉcologie et biologie des interactions [EBI [Poitiers]]
hal.structure.identifierSucres & Échanges Végétaux-Environnement [Équipe du laboratoire EBI Poitiers] [SEVE]
dc.contributor.authorLA CAMERA, Sylvain
hal.structure.identifierÉcologie et biologie des interactions [EBI [Poitiers]]
hal.structure.identifierSucres & Échanges Végétaux-Environnement [Équipe du laboratoire EBI Poitiers] [SEVE]
dc.contributor.authorGAILLARD, Cécile
hal.structure.identifierÉcologie et biologie des interactions [EBI [Poitiers]]
hal.structure.identifierSucres & Échanges Végétaux-Environnement [Équipe du laboratoire EBI Poitiers] [SEVE]
dc.contributor.authorVRIET, Cécile
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorLECOMTE, Pascal
dc.contributor.authorFERRARI, Gérald
hal.structure.identifierÉcologie et biologie des interactions [EBI [Poitiers]]
hal.structure.identifierSucres & Échanges Végétaux-Environnement [Équipe du laboratoire EBI Poitiers] [SEVE]
dc.contributor.authorCOUTOS-THÉVENOT, Pierre
dc.date.accessioned2024-04-08T12:35:42Z
dc.date.available2024-04-08T12:35:42Z
dc.date.issued2019-07-30
dc.identifier.issn1664-462X
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197335
dc.description.abstractEnEutypa lata is the causal agent of eutypa dieback, one of the most destructive grapevine trunk disease that causes severe economic losses in vineyards worldwide. This fungus causes brown sectorial necrosis in wood which affect the vegetative growth. Despite intense research efforts made in the past years, no cure currently exists for this disease. Host responses to eutypa dieback are difficult to address because E. lata is a wood pathogen that causes foliar symptoms several years after infection. With the aim to classify the level of susceptibility of grapevine cultivars to the foliar symptoms caused by E. lata, artificial inoculations of Merlot, Cabernet Sauvignon, and Ugni Blanc were conducted over 3 years. Merlot was the most tolerant cultivar, whereas Ugni Blanc and Cabernet Sauvignon exhibited higher and differential levels of susceptibility. We took advantage of their contrasting phenotypes to explore their defense responses, including the activation of pathogenesis-related (PR) genes, oxylipin and phenylpropanoid pathways and the accumulation of stilbenes. These analyses were carried out using the millicell system that enables the molecular dialogue between E. lata mycelium and grapevine leaves to take place without physical contact. Merlot responded to E. lata by inducing the expression of a large number of defense-related genes. On the contrary, Ugni Blanc failed to activate such defense responses despite being able to perceive the fungus. To gain insight into the role of carbon partitioning in E. lata infected grapevine, we monitored the expression of plant genes involved in sugar transport and cleavage, and measured invertase activities. Our results evidence a coordinated up-regulation of VvHT5 and VvcwINV genes, and a stimulation of the cell wall invertase activity in leaves of Merlot elicited by E. lata, but not in Ugni Blanc. Altogether, this study indicates that the degree of cultivar susceptibility is associated with the activation of host defense responses, including extracellular sucrolytic machinery and hexose uptake during the grapevine/E. lata interaction. Given the role of these activities in governing carbon allocation through the plant, we postulate that the availability of sugar resources for either the host or the fungus is crucial for the outcome of the interaction.
dc.language.isoen
dc.publisherFrontiers
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectEutypa lata
dc.subjectgrape
dc.subject.ensugar transporters
dc.subject.encell wall invertase
dc.subject.encultivar susceptibility
dc.subject.eneutypa dieback
dc.subject.enpathogenesis-related (PR) proteins
dc.subject.enstilbenes
dc.title.enComparison of the Molecular Responses of Tolerant, Susceptible and Highly Susceptible Grapevine Cultivars During Interaction With the Pathogenic Fungus Eutypa lata
dc.typeArticle de revue
dc.identifier.doi10.3389/fpls.2019.00991
dc.subject.halSciences de l'environnement/Biodiversité et Ecologie
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalFrontiers in Plant Science
bordeaux.page1-18
bordeaux.volume10
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02296336
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02296336v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20Plant%20Science&rft.date=2019-07-30&rft.volume=10&rft.spage=1-18&rft.epage=1-18&rft.eissn=1664-462X&rft.issn=1664-462X&rft.au=CARDOT,%20Chlo%C3%A9&MAPPA,%20Ga%C3%ABtan&LA%20CAMERA,%20Sylvain&GAILLARD,%20C%C3%A9cile&VRIET,%20C%C3%A9cile&rft.genre=article


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