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Endophytic bacteria with antagonistic traits inhabit the wood tissues of grapevines from Tunisian vineyards

hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
hal.structure.identifierLaboratoire Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis
dc.contributor.authorREZGUI, Awatef
hal.structure.identifierLaboratoire Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis
dc.contributor.authorBEN GHNAYA-CHAKROUN, A.
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorVALLANCE, Jessica
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorBRUEZ, Emilie
hal.structure.identifierInstitut National de la Recherche Agronomique de Tunisie [INRAT]
dc.contributor.authorHAJLAOUI, M.R.
hal.structure.identifierLaboratoire Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis
dc.contributor.authorSADFI-ZOUAOUI, N.
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorREY, Patrice
dc.date.accessioned2024-04-08T12:32:32Z
dc.date.available2024-04-08T12:32:32Z
dc.date.issued2016
dc.identifier.issn1049-9644
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197144
dc.description.abstractEnVineyards throughout the world, including Tunisia, are being attacked by Grapevine Trunk Diseases (GTDs) such as Esca and Botryosphaeriae dieback. In this study, the bacterial microflora colonizing the non-necrotic and necrotic wood tissues of Tunisian mature grapevines (cv Muscat d’Italie) was investigated. Both types of tissues were studied in order to decipher microbial communities associated with them and to find a suitable BCA that can be applied to the Tunisian terroir. Single-Strand Conformation Polymorphism (SSCP) analyses showed that complex bacterial communities specifically colonized both types of wood tissues. The 19 most abundant cultivable strains, selected on their morphology, were isolated from plant samples and assigned to Pantoea, Pseudomonas, Curtobacterium and Bacillus species based on the 16S rRNA and rpoB genes. Biochemical and microbiological screenings revealed that those 19 strains (i) metabolized differently carbon sources, even within the same species, (ii) possessed antibiotic genes, (iii) produced siderophores and solubilized phosphates and (iv) had an in vitro antagonistic effect against 3 fungal pathogens (Lasidiodiplodia pseudotheobromae, Neofusicoccum parvum and Schizophyllum commune) involved in GTDs. One strain, Bacillus subtilis “B6”, had a positive effect on young vines of a cultivar, Muscat d’Italie, frequently planted in Tunisia, by reducing the size of the wood necrosis caused by N. parvum, showing its potential to counteract infection caused by this GTDs agent.
dc.language.isoen
dc.publisherElsevier
dc.subjectplant protection
dc.subject.enbacterial communities
dc.subject.engrapevine trunk diseases
dc.subject.enfingerprinting method
dc.subject.enwood necrosis
dc.title.enEndophytic bacteria with antagonistic traits inhabit the wood tissues of grapevines from Tunisian vineyards
dc.typeArticle de revue
dc.identifier.doi10.1016/j.biocontrol.2016.04.005
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalBiological Control
bordeaux.page28-37
bordeaux.volume99
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02636781
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02636781v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biological%20Control&rft.date=2016&rft.volume=99&rft.spage=28-37&rft.epage=28-37&rft.eissn=1049-9644&rft.issn=1049-9644&rft.au=REZGUI,%20Awatef&BEN%20GHNAYA-CHAKROUN,%20A.&VALLANCE,%20Jessica&BRUEZ,%20Emilie&HAJLAOUI,%20M.R.&rft.genre=article


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