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Simultaneous quantification of sporangia and zoospores in a biotrophic oomycete with an automatic particle analyzer: Disentangling dispersal and infection potentials

hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDELMAS, Chloé E. L.
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorMAZET, Isabelle D.
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorJOLIVET, Jerome
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDELIERE, Laurent
dc.contributor.authorDELMOTTE, François
dc.date.accessioned2024-04-08T12:32:24Z
dc.date.available2024-04-08T12:32:24Z
dc.date.issued2014
dc.identifier.issn0167-7012
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197137
dc.description.abstractEnQuantitative pathogenicity traits drive the fitness and dynamics of pathogens in agricultural ecosystems and are key determinants of the correct management of crop production over time. However, traits relating to infection potential (i.e. zoospore production) have been less thoroughly investigated in oomycetes than traits relating to dispersal (i.e. sporangium production). We simultaneously quantified sporangium and zoospore production in a biotrophic oomycete, for the joint assessment of life-cycle traits relating to dispersal and infection potentials. We used an automatic particle analyzer to count and size the sporangia and/or zoospores produced at t = 0 min (no zoospore release) and t = 100 min (zoospore release) in 43 Plasmopara viticola isolates growing on the susceptible Vitis vinifera cv. Cabernet Sauvignon. We were able to differentiate and quantify three types of propagules from different stages of the pathogen life cycle: full sporangia, empty sporangia and zoospores. The method was validated by comparing the sporangium and zoospore counts obtained with an automatic particle analyzer and under a stereomicroscope (manual counting). Each isolate produced a mean of 5.8 ± 1.9 (SD) zoospores per sporangium. Significant relationships were found between sporangium production and sporangium size (negative) and between sporangium size and the number of zoospores produced per sporangium (positive). However, there was a significant positive correlation between total sporangium production and total zoospore production. This procedure can provide a valid quantification of the production of both sporangia and zoospores by oomycetes in large numbers of samples, facilitating joint estimation of the dispersal and infection potentials of plant pathogens in various agro-ecological contexts.
dc.language.isoen
dc.publisherElsevier
dc.subjectdispersion
dc.subject.engrapevine downy mildew
dc.subject.enoomycete
dc.subject.ensporangia
dc.subject.enzoospores
dc.title.enSimultaneous quantification of sporangia and zoospores in a biotrophic oomycete with an automatic particle analyzer: Disentangling dispersal and infection potentials
dc.typeArticle de revue
dc.identifier.doi10.1016/j.mimet.2014.10.012
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalJournal of Microbiological Methods
bordeaux.page169-175
bordeaux.volume107
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02637274
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02637274v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Microbiological%20Methods&rft.date=2014&rft.volume=107&rft.spage=169-175&rft.epage=169-175&rft.eissn=0167-7012&rft.issn=0167-7012&rft.au=DELMAS,%20Chlo%C3%A9%20E.%20L.&MAZET,%20Isabelle%20D.&JOLIVET,%20Jerome&DELIERE,%20Laurent&DELMOTTE,%20Fran%C3%A7ois&rft.genre=article


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