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Early detection and identification of larval parasitoids in Lobesia botrana using PCR-RFLP method

hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorPAPURA, Daciana
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorRUSCH, Adrien
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorROUX, Pascale
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDELBAC, Lionel
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorTHIERY, Denis
dc.date.accessioned2024-04-08T12:32:22Z
dc.date.available2024-04-08T12:32:22Z
dc.date.issued2016
dc.identifier.issn1049-9644
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197135
dc.description.abstractEnSeveral larval parasitoid species are natural enemies of the tortricid moths of European vineyards, including the most damaging of these pests, Lobesia botrana. Over the last few years, DNA-based methods have been used for more rapid and accurate detection and identification of parasitoids. In this study, we developed a PCR-RFLP analysis method targeting a mitochondrial cytochrome oxidase I gene fragment after digestion with the restriction enzyme ApoI, for discrimination between four parasitoid species of Lobesia botrana: Campoplex capitator, Exochus tibialis, Elachertus spp. (Hymenoptera, Ichneumonidae) and Phytomyptera nigrina (Diptera, Tachinidae). We assessed the accuracy of this method using populations of L. botrana sampled from eight vineyards located in South-West of France. On a total of 547 L. botrana larvae collected, 252 were analyzed for parasitism using our molecular method whereas the remaining 295 were reared to assess parasitism rates based on emergence. Our PCR-RLFP method showed a mean parasitism rate of 25%, with values ranging from 3% to 50% across vineyards. The levels of parasitism estimated by this method were about three times those estimated after emergence and identification (7.3%). This difference suggests that mortality may occur during parasitoid development, possibly due to encapsulation. Our method revealed that the two dominating parasitoid species were Campoplex capitator (90%) and Phytomyptera nigrina (9%), whereas the emergence of parasitoids found only C. capitator after taxonomical identification. This study revealed that the PCR-RFLP analysis is an appropriate and reliable tool for estimating the biological control potential of a diverse community of parasitoids on the main tortricid moth of grapevine.
dc.language.isoen
dc.publisherElsevier
dc.subject.enhymenoptera parasitoids
dc.subject.engrape vine
dc.subject.enmolecular identification
dc.subject.enPCR-RFLP
dc.subject.enbiological control
dc.title.enEarly detection and identification of larval parasitoids in Lobesia botrana using PCR-RFLP method
dc.typeArticle de revue
dc.identifier.doi10.1016/j.biocontrol.2016.08.005
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences de l'environnement
bordeaux.journalBiological Control
bordeaux.page95-100
bordeaux.volume103
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02637410
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02637410v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biological%20Control&rft.date=2016&rft.volume=103&rft.spage=95-100&rft.epage=95-100&rft.eissn=1049-9644&rft.issn=1049-9644&rft.au=PAPURA,%20Daciana&RUSCH,%20Adrien&ROUX,%20Pascale&DELBAC,%20Lionel&THIERY,%20Denis&rft.genre=article


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