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Screening and modes of action of antagonistic bacteria to control the fungal pathogen Phaeomoniella chlamydospora involved in grapevine trunk diseases

hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
hal.structure.identifierFaculty of Science, Biology Department
dc.contributor.authorHAIDAR, Rana
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorROUDET, Jean
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorBONNARD, Olivier
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDUFOUR, Marie-Cecile
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorCORIO-COSTET, Marie-France
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorFERT, Mathieu
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorGAUTIER, Thomas
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDESCHAMPS, Alain
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorFERMAUD, Marc
dc.date.accessioned2024-04-08T12:32:19Z
dc.date.available2024-04-08T12:32:19Z
dc.date.issued2016
dc.identifier.issn0944-5013
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197133
dc.description.abstractEnThe antagonistic activity of 46 bacterial strains isolated from Bordeaux vineyards were evaluated against Phaeomoniella chlamydospora, a major grapevine pathogen involved in Esca. The reduction of the necrosis length of stem cuttings ranged between 31.4% and 38.7% for the 8 most efficient strains. Two in planta trials allowed the selection of the two best strains, Bacillus pumilus (S32) and Paenibacillus sp. (S19). Their efficacy was not dependent on application method; co-inoculation, prevention in the wood and soil inoculation were tested. The involvement of antibiosis by the secretion of diffusible and/or volatile compounds in the antagonistic capacity of these two strains was assessed in vitro. Volatile compounds secreted by B. pumilus (S32) and Paenibacillus sp. (S19) were identified by gas chromatography/mass spectroscopy (GC/MS). The volatile compounds 1-octen-3-ol and 2,5-dimethyl pyrazine were obtained commercially and tested, and they showed strong antifungal activity against P. chlamydospora, which suggested that these compounds may play an important role in the bacterial antagonistic activity in planta. Furthermore, the expression of 10 major grapevine defense genes was quantified by real-time polymerase chain reaction, which demonstrated that the two strains significantly affected the grapevine transcripts four days after their application on the plants. High expression levels of different genes associated with P. chlamydospora infection in B. pumilus pre-treated plants suggests that this strain induces systemic resistance in grapevine. For the first time, we demonstrated the ability of two bacterial strains, B. pumilus and Paenibacillus sp., isolated from grapevine wood, to control P. chlamydospora via direct and/or indirect mechanisms.
dc.language.isoen
dc.publisherElsevier
dc.title.enScreening and modes of action of antagonistic bacteria to control the fungal pathogen Phaeomoniella chlamydospora involved in grapevine trunk diseases
dc.typeArticle de revue
dc.identifier.doi10.1016/j.micres.2016.07.003
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences de l'environnement
bordeaux.journalMicrobiological Research
bordeaux.page172-184
bordeaux.volume192
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02637680
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02637680v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Microbiological%20Research&rft.date=2016&rft.volume=192&rft.spage=172-184&rft.epage=172-184&rft.eissn=0944-5013&rft.issn=0944-5013&rft.au=HAIDAR,%20Rana&ROUDET,%20Jean&BONNARD,%20Olivier&DUFOUR,%20Marie-Cecile&CORIO-COSTET,%20Marie-France&rft.genre=article


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