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Comparative analysis of expressed CRN and RXLR effectors from two <em>Plasmopara</em> species causing grapevine and sunflower downy mildew

hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorMESTRE ARTIGUES, Pedro-Felipe
hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
dc.contributor.authorCARRERE, Sebastien
hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
dc.contributor.authorGOUZY, Jerome
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorPIRON, Marie-Christine
hal.structure.identifierInstitut National de la Recherche Agronomique [INRA]
dc.contributor.authorTOURVIEILLE DE LABROUHE, Denis
hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
dc.contributor.authorVINCOURT, Patrick
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDELMOTTE, François
hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
dc.contributor.authorGODIARD, Laurence
dc.date.accessioned2024-04-08T12:32:05Z
dc.date.available2024-04-08T12:32:05Z
dc.date.issued2016
dc.identifier.issn0032-0862
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197120
dc.description.abstractEnPlasmopara halstedii and Plasmopara viticola are the causative agents of downy mildew respectively on sunflower and grapevine, two economically important crops. These phylogenetically related oomycetes are obligate biotrophs belonging to Peronosporales but have different pathological profiles. Despite the economic importance of diseases caused by both Plasmopara, genomic resources are very limited and their effector repertoire is unknown. We performed parallel transcriptome sequencing of Pl. halstedii and Pl. viticola and released a Plasmopara species cDNA database (PlasmoparaSp) containing 46 000 clusters. In oomycetes, two classes of effectors are translocated into the host cytoplasm, the RXLRs and the CRNs. We screened the PlasmoparaSp database in order to identify the repertoire of expressed effectors used by both Plasmopara species, about 50 putative RXLR and 60 CRN were identified for each. We compared these effectors within both species and with seven publicly available oomycete species representative of Peronosporales and Albuginales. Sequence analyses revealed the presence of 55 RXLR families, 12 of them shared by both Plasmopara, and 19 showing amino acid conservation with predicted peptides from at least one oomycete species. Analyses of Plasmopara sp. CRN C-terminal variable regions revealed sequence conservation inside Plasmopara sp. and across oomycetes, excepting H. arabidopsidis. Finally, our analyses confirmed the presence of 8 CRN C-terminal domains described in Phytophthora infestans and identified CRN effectors showing similarity to serine proteases. We report effectors identified as species-specific, which may be involved in host specificity, as well as effectors conserved among oomycetes that might play important roles in the pathogens biology.
dc.description.sponsorshipUniversité Fédérale de Toulouse - ANR-11-IDEX-0002
dc.language.isoen
dc.publisherWiley
dc.subjectPlasmopara
dc.subject.enoomycete effectors
dc.subject.endowny mildew
dc.subject.engrapevine
dc.subject.ensunflower
dc.subject.encDNA sequencing
dc.title.enComparative analysis of expressed CRN and RXLR effectors from two <em>Plasmopara</em> species causing grapevine and sunflower downy mildew
dc.typeArticle de revue
dc.identifier.doi10.1111/ppa.12469
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPlant Pathology
bordeaux.page767-781
bordeaux.volume65
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.issue5
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02640035
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02640035v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Plant%20Pathology&amp;rft.date=2016&amp;rft.volume=65&amp;rft.issue=5&amp;rft.spage=767-781&amp;rft.epage=767-781&amp;rft.eissn=0032-0862&amp;rft.issn=0032-0862&amp;rft.au=MESTRE%20ARTIGUES,%20Pedro-Felipe&amp;CARRERE,%20Sebastien&amp;GOUZY,%20Jerome&amp;PIRON,%20Marie-Christine&amp;TOURVIEILLE%20DE%20LABROUHE,%20Denis&amp;rft.genre=article


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