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Assessment of fungicide resistance and pathogen diversity in Erysiphe necator using quantitative real-time PCR assays

hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDUFOUR, Marie-Cecile
hal.structure.identifierAgence Française de Sécurité Sanitaire des Aliments [AFSSA]
dc.contributor.authorFONTAINE, Séverine
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
hal.structure.identifierUnité de Pathologie Végétale [PV]
dc.contributor.authorMONTARRY, Josselin
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorCORIO-COSTET, Marie-France
dc.date.accessioned2024-04-08T12:30:51Z
dc.date.available2024-04-08T12:30:51Z
dc.date.issued2011
dc.identifier.issn1526-498X
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197042
dc.description.abstractEnBACKGROUND: Management of grapevine powdery mildew Erysiphe necator Schw. requires fungicide treatments such as sterol demethylation inhibitors (DMIs) or mitochondrial inhibitors (QoIs). Recently, reduction in the efficacy of DMIs or QoIs was reported in Europe and the United States. The aim of the present study was to develop real-time qPCR tools to detect and quantify several CYP51 gene variants of E. necator: (i) A versus B groups (G37A) and (ii) sensitive versus resistant to sterol demethylase inhibitor fungicides (Y136F). RESULTS: The efficacy of the qPCR tools developed was better than the CAPS method, with a limit of 2 pg for E necator DNA, 0.06 ng for genetic group A and 1.4 ng for the DMI-resistant allele. The detection limits of qPCR protocols (LOD) ranged from 0.72 to 0.85%, and the quantification limits (LOQ) ranged from 2.4 to 2.85% for the two alleles G47A and Y136F respectively. The application of qPCR to field isolates from French vineyards showed the presence of DMI-resistant and/or QoI-resistant alleles in French pathogen populations, linked to genetic group B. CONCLUSION: The real-time PCR assay developed in this study provides a potentially useful tool for efficient quantification of different alleles of interest for fungicide monitoring and for population structure of E. necator.
dc.language.isoen
dc.publisherWiley
dc.rights.urihttp://creativecommons.org/licenses/by-sa/
dc.subjectDMI fungicide
dc.subjectQol
dc.subjectQ-PCR
dc.subject.enbiotype
dc.subject.enpowdery mildew
dc.subject.enresistance
dc.title.enAssessment of fungicide resistance and pathogen diversity in Erysiphe necator using quantitative real-time PCR assays
dc.typeArticle de revue
dc.identifier.doi10.1002/ps.2032
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalPest Management Science
bordeaux.page60-69
bordeaux.volume67
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.issue1
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02652428
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02652428v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Pest%20Management%20Science&rft.date=2011&rft.volume=67&rft.issue=1&rft.spage=60-69&rft.epage=60-69&rft.eissn=1526-498X&rft.issn=1526-498X&rft.au=DUFOUR,%20Marie-Cecile&FONTAINE,%20S%C3%A9verine&MONTARRY,%20Josselin&CORIO-COSTET,%20Marie-France&rft.genre=article


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