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Quantitative trait loci in pepper genome control the effective population size of two RNA viruses at inoculation

hal.structure.identifierUnité de Pathologie Végétale [PV]
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorTAMISIER, Lucie
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
hal.structure.identifierUnité de Pathologie Végétale [PV]
hal.structure.identifierBiological control of artificial ecosystems [BIOCORE]
dc.contributor.authorROUSSEAU, Elsa
hal.structure.identifierUnité de Pathologie Végétale [PV]
dc.contributor.authorBARRAILLÉ, Sebastien
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorNEMOUCHI, Ghislaine
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorSZADKOWSKI, Marion
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
hal.structure.identifierBiological control of artificial ecosystems [BIOCORE]
dc.contributor.authorMAILLERET, Ludovic
hal.structure.identifierBiological control of artificial ecosystems [BIOCORE]
dc.contributor.authorGROGNARD, Frédéric
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorFABRE, Frédéric
hal.structure.identifierUnité de Pathologie Végétale [PV]
dc.contributor.authorMOURY, Benoît
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorPALLOIX, Alain
dc.date.accessioned2024-04-08T12:30:26Z
dc.date.available2024-04-08T12:30:26Z
dc.date.issued2016
dc.date.conference2016-09-12
dc.identifier.isbn978-615-5270-27-7
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197012
dc.description.abstractEnInfection of plants by viruses is a complex process that involves several steps: inoculation into plant cells, replication in inoculated cells, cell-to-cell movement during leaf colonization and long-distance movement during systemic infection. The success of the different steps is conditioned by the effective viral population size (<em>Ne</em>) defined as the number of individuals that pass their genes to the next generation. During the infection cycle, the virus population will endure several bottlenecks leading to drastic reductions in <em>Ne</em> and to the random loss of sorne virus variants. If strong enough, these bottlenecks could act against selection by eliminating the fittest variants. Therefore, a better understanding of how plant affects Ne rnay contribute to the developrnent of durable virus-resistant cultivars. We aimed to (i) identify plant genetic factors that control <em>Ne</em> at the inoculation step, (ii) understand the mechanisms used by the plant to control <em>Ne</em> and (iii) compare these genetic factors with other genes controlling virus life cycle and plant resistance durability. The virus effective population size was measured in a segregating population of 152 doubled-haploid lines of <em>Capsicum annuum</em>. Plants were inoculated mechanically either with a Patata virus Y (PVY) construct expressing the green fluorescent protein (OFP), or a necrotic variant of Cucumber mosaic virus (CMV), the CMV-N strain of Fulton. Ne was assessed by counting the number ofprimary infection foci observed on inoculated cotyledons under UV light for PVY -OFP or the number of necrotic local lesionsobserved on inoculated leaves for CMY-N. The numbers of primary infection foci and locallesions were correlated arnong the doubled-haploid lines (r=0.57) and showed a high heritability (h2=0.93 and 0.98 for PVY and CMV, respectively). The effective population size of the two viruses was shown to be controlled by botb common quantitative trait loci (QTLs) and virus-specifie QTLs, indicating the contribution ofboth general and specifie mechanisms. The PVY-specific QTL colocalizes with a QTL that had previously been shown to be involved in PVY accumulation and capacity to break amajor-effect resistance gene down.
dc.language.isoen
dc.publisherDiamond Congress Ltd
dc.publisher.locationBudapest (hungary)
dc.title.enQuantitative trait loci in pepper genome control the effective population size of two RNA viruses at inoculation
dc.typeCommunication dans un congrès
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.page591 p.
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.conference.title16. Eucarpia Capsicum and Eggplant meeting
bordeaux.countryHU
bordeaux.conference.cityKecskemét
bordeaux.peerReviewednon
hal.identifierhal-02738767
hal.version1
hal.invitednon
hal.conference.organizerSzent István University. Gödöllo, HUN.
hal.conference.end2016-09-14
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02738767v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.date=2016&amp;rft.spage=591%20p.&amp;rft.epage=591%20p.&amp;rft.au=TAMISIER,%20Lucie&amp;ROUSSEAU,%20Elsa&amp;BARRAILL%C3%89,%20Sebastien&amp;NEMOUCHI,%20Ghislaine&amp;SZADKOWSKI,%20Marion&amp;rft.isbn=978-615-5270-27-7&amp;rft.genre=unknown


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