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Survival and ice nucleation activity of bacteria as aerosol in a cloud simulation chamber.

hal.structure.identifierInstitut de Chimie de Clermont-Ferrand [ICCF]
dc.contributor.authorAMATO, Pierre
hal.structure.identifierInstitut de Chimie de Clermont-Ferrand [ICCF]
hal.structure.identifierLaboratoire de météorologie physique [LaMP]
dc.contributor.authorJOLY, M.
hal.structure.identifierInstitute for Meteorology and Climate Research [IMK]
dc.contributor.authorSCHAUPP, C.
hal.structure.identifierInstitut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux [IPREM]
dc.contributor.authorATTARD, Eléonore
hal.structure.identifierInstitute for Meteorology and Climate Research [IMK]
dc.contributor.authorMÖHLER, Ottmar
hal.structure.identifierUnité de Pathologie Végétale [PV]
dc.contributor.authorMORRIS, Cindy E.
hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorBRUNET, Y.
hal.structure.identifierInstitut de Chimie de Clermont-Ferrand [ICCF]
dc.contributor.authorDELORT, A.-M.
dc.date.accessioned2024-04-08T12:11:13Z
dc.date.available2024-04-08T12:11:13Z
dc.date.issued2015
dc.identifier.issn1680-7316
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/196681
dc.description.abstractEnThe residence time of bacterial cells in the atmosphere is predictable by numerical models. However, estimations of their aerial dispersion as living entities are limited by a lack of information concerning survival rates and behavior in relation to atmospheric water. Here we investigate the viability and ice nucleation (IN) activity of typical atmospheric ice nucleation active bacteria (Pseudomonas syringae and P. fluorescens) when airborne in a cloud simulation chamber (AIDA, Karlsruhe, Germany). Cell suspensions were sprayed into the chamber and aerosol samples were collected by impingement at designated times over a total duration of up to 18 h, and at some occasions after dissipation of a cloud formed by depressurization. Aerosol concentration was monitored simultaneously by online instruments. The cultivability of airborne cells decreased exponentially over time with a half-life time of 250 ± 30 min (about 3.5 to 4.5 h). In contrast, IN activity remained unchanged for several hours after aerosolization, demonstrating that IN activity was maintained after cell death. Interestingly, the relative abundance of IN active cells still airborne in the chamber was strongly decreased after cloud formation and dissipation. This illustrates the preferential precipitation of IN active cells by wet processes. Our results indicate that from 106 cells aerosolized from a surface, one would survive the average duration of its atmospheric journey estimated at 3.4 days. Statistically, this corresponds to the emission of 1 cell that achieves dissemination every ~ 33 min m−2 of cultivated crops fields, a strong source of airborne bacteria. Based on the observed survival rates, depending on wind speed, the trajectory endpoint could be situated several hundreds to thousands of kilometers from the emission source. These results should improve the representation of the aerial dissemination of bacteria in numeric models.
dc.language.isoen
dc.publisherEuropean Geosciences Union
dc.title.enSurvival and ice nucleation activity of bacteria as aerosol in a cloud simulation chamber.
dc.typeArticle de revue
dc.identifier.doi10.5194/acp-15-6455-2015
dc.subject.halChimie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie
dc.subject.halSciences de l'environnement/Biodiversité et Ecologie
bordeaux.journalAtmospheric Chemistry and Physics
bordeaux.page4055-4082
bordeaux.volume15
bordeaux.hal.laboratoriesInteractions Soil Plant Atmosphere (ISPA) - UMR 1391*
bordeaux.issue11
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-01206705
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01206705v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Atmospheric%20Chemistry%20and%20Physics&rft.date=2015&rft.volume=15&rft.issue=11&rft.spage=4055-4082&rft.epage=4055-4082&rft.eissn=1680-7316&rft.issn=1680-7316&rft.au=AMATO,%20Pierre&JOLY,%20M.&SCHAUPP,%20C.&ATTARD,%20El%C3%A9onore&M%C3%96HLER,%20Ottmar&rft.genre=article


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