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Non-destructive estimates of soil carbonic anhydrase activity and associated soil water oxygen isotope composition

hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorJONES, Samuel P.
hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorOGÉE, Jérôme
hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorSAUZE, Joana
hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorWOHL, Steven
hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorSAAVEDRA, Noelia
hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorFERNÁNDEZ-PRADO, Noelia
hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorMAIRE, Juliette
hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorLAUNOIS, Thomas
hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorBOSC, Alexandre
hal.structure.identifierInteractions Sol Plante Atmosphère [UMR ISPA]
dc.contributor.authorWINGATE, Lisa
dc.date.accessioned2024-04-08T12:06:19Z
dc.date.available2024-04-08T12:06:19Z
dc.date.issued2017
dc.identifier.issn1027-5606
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/196396
dc.description.abstractEnThe contribution of photosynthesis and soil respiration to net land-atmosphere carbon dioxide (CO2) exchange can be estimated based on the differential influence of leaves and soils on budgets of the oxygen isotope composition (delta O-18) of atmospheric CO2. To do so, the activity of carbonic anhydrases (CAs), a group of enzymes that catalyse the hydration of CO2 in soils and plants, needs to be understood. Measurements of soil CA activity typically involve the inversion of models describing the delta O-18 of CO2 fluxes to solve for the apparent, potentially catalysed, rate of CO2 hydration. This requires information about the delta O-18 of CO2 in isotopic equilibrium with soil water, typically obtained from destructive, depth-resolved sampling and extraction of soil water. In doing so, an assumption is made about the soil water pool that CO2 interacts with, which may bias estimates of CA activity if incorrect. Furthermore, this can represent a significant challenge in data collection given the potential for spatial and temporal variability in the delta O-18 of soil water and limited a priori information with respect to the appropriate sampling resolution and depth. We investigated whether we could circumvent this requirement by inferring the rate of CO2 hydration and the delta O-18 of soil water from the relationship between the delta O-18 of CO2 fluxes and the delta O-18 of CO2 at the soil surface measured at different ambient CO2 conditions. This approach was tested through laboratory incubations of air-dried soils that were re-wetted with three waters of different delta O-18. Gas exchange measurements were made on these soils to estimate the rate of hydration and the delta O-18 of soil water, followed by soil water extraction to allow for comparison. Estimated rates of CO2 hydration were 6.8-14.6 times greater than the theoretical uncatalysed rate of hydration, indicating that CA were active in these soils. Importantly, these estimates were not significantly different among water treatments, suggesting that this represents a robust approach to assay the activity of CA in soil. As expected, estimates of the delta O-18 of the soil water that equilibrates with CO2 varied in response to alteration to the delta O-18 of soil water. However, these estimates were consistently more negative than the composition of the soil water extracted by cryogenic vacuum distillation at the end of the gas measurements with differences of up to 3.94% VSMOW-SLAP. These offsets suggest that, at least at lower water contents, CO2-H2O isotope equilibration primarily occurs with water pools that are bound to particle surfaces and are depleted in O-18 compared to bulk soil water.
dc.language.isoen
dc.publisherEuropean Geosciences Union
dc.title.enNon-destructive estimates of soil carbonic anhydrase activity and associated soil water oxygen isotope composition
dc.typeArticle de revue
dc.identifier.doi10.5194/hess-21-6363-2017
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences de l'environnement
bordeaux.journalHydrology and Earth System Sciences
bordeaux.page6363-6377
bordeaux.volume21
bordeaux.hal.laboratoriesInteractions Soil Plant Atmosphere (ISPA) - UMR 1391*
bordeaux.issue12
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02620542
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02620542v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Hydrology%20and%20Earth%20System%20Sciences&rft.date=2017&rft.volume=21&rft.issue=12&rft.spage=6363-6377&rft.epage=6363-6377&rft.eissn=1027-5606&rft.issn=1027-5606&rft.au=JONES,%20Samuel%20P.&OG%C3%89E,%20J%C3%A9r%C3%B4me&SAUZE,%20Joana&WOHL,%20Steven&SAAVEDRA,%20Noelia&rft.genre=article


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