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Identification of ion currents components generating field potential recorded in MEA from hiPSC-CM

hal.structure.identifierNumerical simulation of biological flows [REO]
dc.contributor.authorRAPHEL, Fabien
hal.structure.identifierNumerical simulation of biological flows [REO]
dc.contributor.authorBOULAKIA, Muriel
hal.structure.identifierModélisation et calculs pour l'électrophysiologie cardiaque [CARMEN]
dc.contributor.authorZEMZEMI, Nejib
hal.structure.identifierModélisation et calculs pour l'électrophysiologie cardiaque [CARMEN]
dc.contributor.authorCOUDIÈRE, Yves
hal.structure.identifierSanofi-Aventis R&D
dc.contributor.authorGUILLON, Jean-Michel
hal.structure.identifierLIPZ [La Celle Saint-Cloud]
dc.contributor.authorZITOUN, Philippe
hal.structure.identifierNumerical simulation of biological flows [REO]
dc.contributor.authorGERBEAU, Jean-Frédéric
dc.date.accessioned2024-04-04T03:09:25Z
dc.date.available2024-04-04T03:09:25Z
dc.date.issued2018
dc.identifier.issn0018-9294
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/193603
dc.description.abstractEnObjective: Multi Electrodes Arrays (MEAs) combined with cardiomy-ocytes derived from human induced pluripotent stem cells (hiPSC-CMs) can enable high-or medium-throughput drug screening in safety pharma-cology. This technology has recently attracted a lot of attention, in particular from an international initiative named CiPA. But it is currently limited by the difficulty to analyze the measured signals. We propose a strategy to analyze the signals acquired by the MEA and to automatically deduce the channels affected by the drug. Methods: Our method is based on the bidomain equations, a model for the MEA electrodes, and an inverse problem strategy. Results: In silico MEA signals are obtained for two commercial devices and an example of Early After Depolarization (EAD) is presented. Then, by processing real signals obtained for four different compounds, our algorithm was able to provide dose-response curves for potassium, sodium and calcium channels. For ivabradine and moxifloxacin, the IC50 and dose-response curves are in very good agreement with known values. Significance: The proposed strategy offers a possible answer to a major question raised by the community of safety pharmacology. By allowing a more automated analysis of the signals, our approach could contribute to promote the technology based on MEA and hiPSC-CMs, and therefore improve reliability and efficiency of drug screening.
dc.description.sponsorshipModélisation, simulation et traitement du signal en électrophysiologie cardiaque - ANR-13-LAB1-0007
dc.description.sponsorshipAgency for mathematics in interaction with enterprise and society - ANR-10-LABX-0002
dc.language.isoen
dc.publisherInstitute of Electrical and Electronics Engineers
dc.subject.enCardiac Electrophysiology.
dc.subject.enMulti Electrodes Arrays
dc.subject.enSafety pharmacology
dc.title.enIdentification of ion currents components generating field potential recorded in MEA from hiPSC-CM
dc.typeArticle de revue
dc.identifier.doi10.1109/TBME.2017.2748798
dc.subject.halMathématiques [math]/Analyse numérique [math.NA]
dc.subject.halSciences du Vivant [q-bio]/Sciences pharmaceutiques/Pharmacologie
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologie/Cardiologie et système cardiovasculaire
bordeaux.journalIEEE Transactions on Biomedical Engineering
bordeaux.page1311-1319
bordeaux.volume65
bordeaux.hal.laboratoriesInstitut de Mathématiques de Bordeaux (IMB) - UMR 5251*
bordeaux.issue6
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-01570341
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01570341v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=IEEE%20Transactions%20on%20Biomedical%20Engineering&rft.date=2018&rft.volume=65&rft.issue=6&rft.spage=1311-1319&rft.epage=1311-1319&rft.eissn=0018-9294&rft.issn=0018-9294&rft.au=RAPHEL,%20Fabien&BOULAKIA,%20Muriel&ZEMZEMI,%20Nejib&COUDI%C3%88RE,%20Yves&GUILLON,%20Jean-Michel&rft.genre=article


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