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Tracking of Cell Nuclei for Assessment of In Vitro Uptake Kinetics in Ultrasound-Mediated Drug Delivery using Fibered Confocal Fluorescence Microscopy

hal.structure.identifierImage sciences institute - University of Utrecht [ISI]
dc.contributor.authorDERIEPPE, Marc
hal.structure.identifierImage sciences institute - University of Utrecht [ISI]
hal.structure.identifierInstitut de Mathématiques de Bordeaux [IMB]
dc.contributor.authorDENIS DE SENNEVILLE, Baudouin
hal.structure.identifierImage sciences institute - University of Utrecht [ISI]
dc.contributor.authorKUIJF, Hugo
hal.structure.identifierImage sciences institute - University of Utrecht [ISI]
dc.contributor.authorMOONEN, Chrit
hal.structure.identifierImage sciences institute - University of Utrecht [ISI]
dc.contributor.authorBOS, Clemens
dc.date.accessioned2024-04-04T03:09:10Z
dc.date.available2024-04-04T03:09:10Z
dc.date.issued2014
dc.identifier.issn1536-1632
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/193585
dc.description.abstractEnPurpose: Previously, we demonstrated the feasibility to monitor in real-time the ultrasound-mediated uptake of a cell-impermeable model drug with fibered confocal fluorescence microscopy. Here we present a complete post-processing methodology in order to improve the accuracy of the measured pharmacokinetic parameters.Procedures: After the detection of the nuclei based on a radial symmetry transform algorithm, a frame-by-frame tracking allowed for the monitoring of each individual uptake. The resulting pharmacokinetic parameters were derived from a two-compartment model.Results: With the tracking, 93% of the 370 nuclei showed a fluorescence signal variation that was well described by a two-compartment model. For each kinetic parameter, the tracking allowed for more homogeneous distributions.Conclusions: This post-processing methodology improved the accuracy of the uptake pharmacokinetic parameters assessed in a cell population. This study extends the proof of concept of designing an in vitro setup for the real-time monitoring of an US-mediated model drug uptake.
dc.language.isoen
dc.publisherSpringer Verlag
dc.title.enTracking of Cell Nuclei for Assessment of In Vitro Uptake Kinetics in Ultrasound-Mediated Drug Delivery using Fibered Confocal Fluorescence Microscopy
dc.typeArticle de revue
dc.identifier.doi10.1007/s11307-014-0726-3
dc.subject.halSciences de l'ingénieur [physics]/Traitement du signal et de l'image
dc.subject.halInformatique [cs]/Traitement du signal et de l'image
dc.subject.halInformatique [cs]/Imagerie médicale
dc.subject.halSciences du Vivant [q-bio]/Sciences pharmaceutiques/Pharmacologie
bordeaux.journalMolecular Imaging and Biology
bordeaux.page642-651
bordeaux.volume16
bordeaux.hal.laboratoriesInstitut de Mathématiques de Bordeaux (IMB) - UMR 5251*
bordeaux.issue5
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-00994166
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00994166v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20Imaging%20and%20Biology&rft.date=2014&rft.volume=16&rft.issue=5&rft.spage=642-651&rft.epage=642-651&rft.eissn=1536-1632&rft.issn=1536-1632&rft.au=DERIEPPE,%20Marc&DENIS%20DE%20SENNEVILLE,%20Baudouin&KUIJF,%20Hugo&MOONEN,%20Chrit&BOS,%20Clemens&rft.genre=article


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