Afficher la notice abrégée

Recruitment of endocytosis in sonopermeabilization-mediated drug delivery: A real-time study

hal.structure.identifierUniversity Medical Center [Utrecht] [UMCU]
dc.contributor.authorDERIEPPE, Marc
hal.structure.identifierUniversity Medical Center [Utrecht] [UMCU]
dc.contributor.authorROJEK, Katarzyna
hal.structure.identifierUniversity Medical Center [Utrecht] [UMCU]
dc.contributor.authorESCOFFRE, Jean-Michel
hal.structure.identifierUniversity Medical Center [Utrecht] [UMCU]
hal.structure.identifierInstitut de Mathématiques de Bordeaux [IMB]
dc.contributor.authorDENIS DE SENNEVILLE, Baudouin
hal.structure.identifierUniversity Medical Center [Utrecht] [UMCU]
dc.contributor.authorMOONEN, Chrit
hal.structure.identifierUniversity Medical Center [Utrecht] [UMCU]
dc.contributor.authorBOS, Clemens
dc.date.accessioned2024-04-04T03:08:57Z
dc.date.available2024-04-04T03:08:57Z
dc.date.issued2015-03-01
dc.identifier.issn1478-3967
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/193572
dc.description.abstractEnMicrobubbles (MB) in combination with ultrasound (US) can enhance cell membrane permeability, and have the potential to facilitate the cellular uptake of hydrophilic molecules. However, the exact mechanism behind US- and MB-mediated intracellular delivery still remains to be fully understood. Among the proposed mechanisms are formation of transient pores and endocytosis stimulation. In our study, we investigated whether endocytosis is involved in US- and MB- mediated delivery of small molecules.Dynamic fluorescence microscopy was used to investigate the effects of endocytosis inhibitors on the pharmacokinetic parameters of US- and MB-mediated uptake of SYTOX Green, a 600 Da hydrophilic model drug. C6 rat glioma cells, together with SonoVue® microbubbles, were exposed to 1.4 MHz ultrasound waves at 0.2 MPa peak-negative pressure. Collection of the signal intensity in each individual nucleus was monitored during and after US exposure by a fibered confocal fluorescence microscope designed for real-time imaging.Exposed to ultrasound waves, C6 cells pretreated with chlorpromazine, an inhibitor of clathrin-mediated endocytosis, showed up to a 2.5-fold significant increase of the uptake time constant, and a 1.1-fold increase with genistein, an inhibitor of caveolae-mediated endocytosis. Both inhibitors slowed down the US-mediated uptake of SYTOX Green.With C6 cells and our experimental settings, these quantitative data indicate that endocytosis plays a role in sonopermeabilization-mediated delivery of small molecules with a more predominant contribution of clathrin-mediated endocytosis.
dc.language.isoen
dc.publisherInstitute of Physics: Hybrid Open Access
dc.title.enRecruitment of endocytosis in sonopermeabilization-mediated drug delivery: A real-time study
dc.typeArticle de revue
dc.subject.halSciences de l'ingénieur [physics]/Traitement du signal et de l'image
bordeaux.journalPhysical Biology
bordeaux.page-
bordeaux.hal.laboratoriesInstitut de Mathématiques de Bordeaux (IMB) - UMR 5251*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-01159814
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01159814v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Physical%20Biology&rft.date=2015-03-01&rft.spage=-&rft.epage=-&rft.eissn=1478-3967&rft.issn=1478-3967&rft.au=DERIEPPE,%20Marc&ROJEK,%20Katarzyna&ESCOFFRE,%20Jean-Michel&DENIS%20DE%20SENNEVILLE,%20Baudouin&MOONEN,%20Chrit&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée